ArticleCell cycle (Georgetown, Tex.)2008
Yeast Chfr homologs retard cell cycle at G1 and G2/M via Ubc4 and Ubc13/Mms2-dependent ubiquitination.
Article in Cell cycle (Georgetown, Tex.), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- Spatiotemporal regulation of the Dma1-mediated mitotic checkpoint coordinates mitosis with cytokinesis.Current genetics · 2019Review
- Ubc13: the Lys63 ubiquitin chain building machine.Oncotarget · 2016Review
- Control of Formin Distribution and Actin Cable Assembly by the E3 Ubiquitin Ligases Dma1 and Dma2.Genetics · 2016Article
- UbiSite: incorporating two-layered machine learning method with substrate motifs to predict ubiquitin-conjugation site on lysines.BMC systems biology · 2016Article
- Bioinformatics analysis identifies several intrinsically disordered human E3 ubiquitin-protein ligases.PeerJ · 2016Article
- Release from myosin V via regulated recruitment of an E3 ubiquitin ligase controls organelle localization.Developmental cell · 2014Article
- Saccharomyces cerevisiae Dma proteins participate in cytokinesis by controlling two different pathways.Cell cycle (Georgetown, Tex.) · 2013Article
- Dma/RNF8 proteins are evolutionarily conserved E3 ubiquitin ligases that target septins.Cell cycle (Georgetown, Tex.) · 2013Article
- Defective in mitotic arrest 1 (Dma1) ubiquitin ligase controls G1 cyclin degradation.The Journal of biological chemistry · 2013Article
- Dnt1 acts as a mitotic inhibitor of the spindle checkpoint protein dma1 in fission yeast.Molecular biology of the cell · 2012Article
- Budding yeast dma proteins control septin dynamics and the spindle position checkpoint by promoting the recruitment of the Elm1 kinase to the bud neck.PLoS genetics · 2012Article
- Uncovering ubiquitin and ubiquitin-like signaling networks.Chemical reviews · 2011Review
- Substrate analysis of the Pneumocystis carinii protein kinases PcCbk1 and PcSte20 using yeast proteome microarrays provides a novel method for Pneumocystis signalling biology.Yeast (Chichester, England) · 2011Article
- The auto-ubiquitylation of E3 ubiquitin-protein ligase Chfr at G2 phase is required for accumulation of polo-like kinase 1 and mitotic entry in mammalian cells.The Journal of biological chemistry · 2011Article
- Budding yeast Dma1 and Dma2 participate in regulation of Swe1 levels and localization.Molecular biology of the cell · 2011Article
- Dma1 ubiquitinates the SIN scaffold, Sid4, to impede the mitotic localization of Plo1 kinase.The EMBO journal · 2011Article
- Article
- The multiple layers of ubiquitin-dependent cell cycle control.Chemical reviews · 2009Review
- Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Checkpoint with forkhead-associated and RING (Chfr) is a ubiquitin ligase (E3) that establishes an antephase or prometaphase checkpoint in response to mitotic stress. Though ubiquitination is essential for checkpoint function, the sites, linkages and ubiquitin conjugating enzyme (E2) specificity are controversial. Here we dissect the function of the two Chfr homologs in S. cerevisiae, Chf1 and Chf2, overexpression of which retard cell cycle at both G(1) and G(2). Using a genetic assay, we establish that Ubc4 is required for Chf2-dependent G(1) cell cycle delay and Chf protein turnover. In contrast, Ubc13/Mms2 is required for G(2) delay and does not contribute to Chf protein turnover. By reconstituting cis and trans-ubiquitination activities of Chf proteins in purified systems and characterizing sites modified and linkages formed by tandem mass spectrometry, we discovered that Ubc13/Mms2- dependent modifications are a distinct subset of those catalyzed by Ubc4. Mutagenesis of Lys residues identified in vitro indicates that site-specific Ubc4-dependent Chf protein autoubiquitination is responsible for Chf protein turnover. Thus, combined genetic and biochemical analyses indicate that Chf proteins have dual E2 specificity accounting for different functions in the cell cycle.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.