ArticleGenetics2008
Dual involvement of CbrAB and NtrBC in the regulation of histidine utilization in Pseudomonas fluorescens SBW25.
Article in Genetics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers, 1 of them a synthesis that pooled it.
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Who cites it
46 citing papers in PubMed, 1 synthesis or guideline pooled it, 78 citations in OpenAlex.
- Implications of carbon catabolite repression for plant-microbe interactions.Plant communications · 2022Pooled it
- Structure and conformational dynamics of the Pseudomonas CbrA transceptor.Protein science : a publication of the Protein Society · 2026Article
- Structure and conformational dynamics of thebioRxiv : the preprint server for biology · 2026Article
- The link of carbon catabolite repression elements, small RNAs CrcY and CrcZ and polyhydroxyalkanoate metabolism in Pseudomonas putida KT2440.Biotechnology for biofuels and bioproducts · 2025Article
- Opening the Random Forest Black Box ofMetabolites · 2023Article
- Mutational hotspots lead to robust but suboptimal adaptive outcomes in certain environments.Microbiology (Reading, England) · 2023Article
- Article
- Insight rifampicin-resistant (rpoB) mutation in Pseudomonas stutzeri leads to enhance the biosynthesis of secondary metabolites to survive against harsh environments.Archives of microbiology · 2022Article
- 2-Aminoethylphosphonate utilization in Pseudomonas putida BIRD-1 is controlled by multiple master regulators.Environmental microbiology · 2022Article
- Review
- Role of Two-Component System Networks in Pseudomonas aeruginosa Pathogenesis.Advances in experimental medicine and biology · 2022Article
- RpuS/R Is a Novel Two-Component Signal Transduction System That Regulates the Expression of the Pyruvate Symporter MctP inFrontiers in microbiology · 2022Article
- Master regulator NtrC controls the utilization of alternative nitrogen sources in Pseudomonas stutzeri A1501.World journal of microbiology & biotechnology · 2021Article
- The Connection between Czc and Cad Systems Involved in Cadmium Resistance inInternational journal of molecular sciences · 2021Article
- Involvement of the MxtR/ErdR (CrbS/CrbR) Two-Component System in Acetate Metabolism inMicroorganisms · 2021Article
- A Novel Marine Pathogen Isolated from Wild Cunners (Microorganisms · 2021Article
- Role of a local transcription factor in governing cellular carbon/nitrogen homeostasis in Pseudomonas fluorescens.Nucleic acids research · 2021Article
- Histidine Utilization Is a Critical Determinant ofInfection and immunity · 2020Article
- Global Regulatory Roles of the Histidine-Responsive Transcriptional Repressor HutC in Pseudomonas fluorescens SBW25.Journal of bacteriology · 2020Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pseudomonas fluorescens SBW25 is capable of growing on histidine as a sole source of carbon and/or nitrogen. Previous work showed that the two-component regulatory system CbrAB is required for expression of the histidine utilization (hut) locus when histidine is the sole source of carbon and nitrogen. Here, using mutational analysis and transcriptional assays, we demonstrate involvement of a second two-component system, NtrBC. When histidine is the sole carbon source, transcription of the hutU operon is initiated from a sigma54-type promoter and requires CbrB (an enhancer binding protein for sigma54-recruitment). However, when histidine is the sole nitrogen source, the hutU operon is transcribed from a sigma70-type promoter and requires either CbrB or the nitrogen regulator, NtrC. No role was found for the SBW25 homolog of the nitrogen assimilation control protein (NAC). Biolog phenotypic microarray analysis of the ability of the three mutants (DeltacbrB, DeltantrC, and DeltacbrB DeltantrC) to utilize 190 carbon and 95 nitrogen substrates confirmed the central regulatory roles of CbrAB and NtrBC in cellular carbon and nitrogen catabolism: deletion of cbrB abolished growth on 20 carbon substrates; deletion of ntrC eliminated growth on 28 nitrogen substrates. A double cbrB-ntrC mutant was unable to utilize a further 14 nitrogen substrates (including histidine, proline, leucine, isoleucine, and valine). Our data show that CbrAB plays a role in regulation of both carbon and nitrogen catabolism and maintains activity of catabolic pathways under different C:N ratios.
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