ArticleGene expression1991
Effects of long 5' leader sequences on initiation by eukaryotic ribosomes in vitro.
Article in Gene expression, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
53 citing papers in PubMed.
- 40S ribosomal subunits move along the 5' UTR through an eIF4A-independent mechanism in higher eukaryotes.bioRxiv : the preprint server for biology · 2026Article
- 40S ribosomal subunits scan mRNA for the start codon by one-dimensional diffusion.RNA (New York, N.Y.) · 2025Article
- The regulatory landscape of 5' UTRs in translational control during zebrafish embryogenesis.Developmental cell · 2025Article
- The regulatory landscape of 5' UTRs in translational control during zebrafish embryogenesis.bioRxiv : the preprint server for biology · 2023Article
- De novo genes with an lncRNA origin encode unique human brain developmental functionality.Nature ecology & evolution · 2023Article
- mRNA-Based Vaccines and Mode of Action.Current topics in microbiology and immunology · 2022Review
- Optimization of translation enhancing element use to increase protein expression in a vaccinia virus system.The Journal of general virology · 2021Article
- Differential processing and localization of human Nocturnin controls metabolism of mRNA and nicotinamide adenine dinucleotide cofactors.The Journal of biological chemistry · 2020Article
- Toward a Kinetic Understanding of Eukaryotic Translation.Cold Spring Harbor perspectives in biology · 2019Review
- Dynamic Interaction of Eukaryotic Initiation Factor 4G1 (eIF4G1) with eIF4E and eIF1 Underlies Scanning-Dependent and -Independent Translation.Molecular and cellular biology · 2018Article
- Efficient and Accurate Translation Initiation Directed by TISU Involves RPS3 and RPS10e Binding and Differential Eukaryotic Initiation Factor 1A Regulation.Molecular and cellular biology · 2017Article
- RAN translation-What makes it run?Brain research · 2016Review
- Ribosomal 18S rRNA base pairs with mRNA during eukaryotic translation initiation.Nature communications · 2016Article
- Human dipeptidyl peptidase III mRNA variant I and II are expressed concurrently in multiple tumor derived cell lines and translated at comparable efficiency in vitro.Molecular biology reports · 2016Article
- Translation initiation of the HIV-1 mRNA.Translation (Austin, Tex.) · 2014Review
- Co-occurrence of transcription and translation gene regulatory features underlies coordinated mRNA and protein synthesis.BMC genomics · 2014Article
- Translation of human tissue factor pathway inhibitor-β mRNA is controlled by alternative splicing within the 5' untranslated region.Arteriosclerosis, thrombosis, and vascular biology · 2014Article
- Transcript mapping of Cotton leaf curl Burewala virus and its cognate betasatellite, Cotton leaf curl Multan betasatellite.Virology journal · 2012Article
- Molecular mechanism of scanning and start codon selection in eukaryotes.Microbiology and molecular biology reviews : MMBR · 2011Review
- Unique translation initiation of mRNAs-containing TISU element.Nucleic acids research · 2011Article
Corrections and comments
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Authors and funding
1 author.
Funding
Abstract
Lengthening the 5' noncoding sequence on SP6-derived transcripts can increase their translational efficiency by an order of magnitude under some conditions of translation in reticulocyte lysates. This effect was observed upon reiterating three different synthetic oligonucleotides, the sequences of which were designed simply to preclude secondary structure. It seems unlikely that such arbitrarily designed sequences are recognized by sequence-specific translational enhancer proteins. Rather, long 5' leader sequences appear to accumulate extra 40S ribosomal subunits, which may account for their translational advantage. The buildup of 40S subunits on long, unstructured leader sequences is predicted by the scanning model for initiation. Leader sequences such as these may be ideal for in vitro expression vectors.
Indexed as
Identifiers
1820209PMC5952206What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.