ArticleExperimental neurology2008
Activated polymorphonuclear cells promote injury and excitability of dorsal root ganglia neurons.
Article in Experimental neurology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- Prenatal Alcohol Exposure: Impact on Neuroimmune Function in the Stress Response and Pain.Advances in experimental medicine and biology · 2026Review
- Neutrophils in Rat Dorsal Root Ganglion during Acute Systemic Inflammation.Bulletin of experimental biology and medicine · 2026Article
- A potential therapeutic target: The role of neutrophils in the central nervous system.Brain, behavior, & immunity - health · 2023Review
- Neutrophil biology in injuries and diseases of the central and peripheral nervous systems.Progress in neurobiology · 2023Review
- Neutrophils infiltrate sensory ganglia and mediate chronic widespread pain in fibromyalgia.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Neutrophil Heterogeneity and its Roles in the Inflammatory Network after Ischemic Stroke.Current neuropharmacology · 2023Article
- Characterization of neutrophil-neuronal co-cultures to investigate mechanisms of post-ischemic immune-mediated neurotoxicity.Journal of neuroscience methods · 2020Article
- The Neuropathic Itch Caused by Pseudorabies Virus.Pathogens (Basel, Switzerland) · 2020Review
- Virulent Pseudorabies Virus Infection Induces a Specific and Lethal Systemic Inflammatory Response in Mice.Journal of virology · 2018Article
- Neuroimmune-Glia Interactions in the Sensory Ganglia Account for the Development of Acute Herpetic Neuralgia.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Role of lipocalin-2-chemokine axis in the development of neuropathic pain following peripheral nerve injury.The Journal of biological chemistry · 2013Article
- Article
- DNA damage response in peripheral nervous system: coping with cancer therapy-induced DNA lesions.DNA repair · 2013Review
- Neutrophil cerebrovascular transmigration triggers rapid neurotoxicity through release of proteases associated with decondensed DNA.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- TRPM2 contributes to inflammatory and neuropathic pain through the aggravation of pronociceptive inflammatory responses in mice.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Neutrophil responses to injury or inflammation impair peripheral gustatory function.Neuroscience · 2010Article
- Inflammation and the neurovascular unit in the setting of focal cerebral ischemia.Neuroscience · 2009Review
- Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
Abstract
Therapies aimed at depleting or blocking the migration of polymorphonuclear leukocytes (PMN or neutrophils) are partially successful in the treatment of neuroinflammatory conditions and in attenuating pain following peripheral nerve injury or subcutaneous inflammation. However, the functional effects of PMN on peripheral sensory neurons such as dorsal root ganglia (DRG) neurons are largely unknown. We hypothesized that PMN are detrimental to neuronal viability in culture and increase neuronal activity and excitability. We demonstrate that isolated peripheral PMN are initially in a relatively resting state but undergo internal oxidative burst and activation by an unknown mechanism within 10 min of co-culture with dissociated DRG cells. Co-culture for 24 h decreases neuronal count at a threshold<0.4:1 PMN:DRG cell ratio and increases the number of injured and apoptotic neurons. Within 3 min of PMN addition, fluorometric calcium imaging reveals intracellular calcium transients in small size (<25 microm diam) and large size (>25 microm diam) neurons, as well as in capsaicin-sensitive neurons. Furthermore, small size isolectin B4-labeled neurons undergo hyperexcitability manifested as decreased current threshold and increased firing frequency. Although co-culture of PMN and DRG cells does not perfectly model neuroinflammatory conditions in vivo, these findings suggest that activated PMN can potentially aggravate neuronal injury and cause functional changes to peripheral sensory neurons. Distinguishing the beneficial from the detrimental effects of PMN on neurons may aid in the development of more effective drug therapies for neurological disorders involving neuroinflammation, including painful neuropathies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.