ArticleWorld journal of gastroenterology2008
Inhibition of signal transducer and activator of transcription 3 expression by RNA interference suppresses invasion through inducing anoikis in human colon cancer cells.
Article in World journal of gastroenterology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
- Metabolic reprogramming and signaling adaptations in anoikis resistance: mechanisms and therapeutic targets.Molecular and cellular biochemistry · 2025Review
- Lichen-Derived Depsides and Depsidones Modulate the Nrf2, NF-κB and STAT3 Signaling Pathways in Colorectal Cancer Cells.Molecules (Basel, Switzerland) · 2021Article
- Treatment of Colon Cancer by Degradable rrPPC Nano-Conjugates Delivered STAT3 siRNA.International journal of nanomedicine · 2020Article
- Feng-Liao-Chang-Wei-Kang Combined with 5-Fluorouracil Synergistically Suppresses Colitis-Associated Colorectal Cancer via the IL-6/STAT3 Signalling Pathway.Evidence-based complementary and alternative medicine : eCAM · 2020Article
- Clinicopathological significance and prognostic role of p-STAT3 in patients with hepatocellular carcinoma.OncoTargets and therapy · 2018Article
- STAT3 activation in infection and infection-associated cancer.Molecular and cellular endocrinology · 2017Review
- Rational combination of MEK inhibitor and the STAT3 pathway modulator for the therapy in K-Ras mutated pancreatic and colon cancer cells.Oncotarget · 2015Article
- ING4 enhances paclitaxel's effect on colorectal cancer growth in vitro and in vivo.International journal of clinical and experimental pathology · 2015Article
- STAT3 and sphingosine-1-phosphate in inflammation-associated colorectal cancer.World journal of gastroenterology · 2014Review
- Dual Aurora A and JAK2 kinase blockade effectively suppresses malignant transformation.Oncotarget · 2014Article
- Uncovering a key to the process of metastasis in human cancers: a review of critical regulators of anoikis.Journal of cancer research and clinical oncology · 2013Review
- Suppression of metastasis of human pancreatic cancer cells to the liver by small interfering RNA-mediated targeting of the midkine gene.Oncology letters · 2013Article
- Deficiency of Erbin induces resistance of cervical cancer cells to anoikis in a STAT3-dependent manner.Oncogenesis · 2013Article
- Beneficial effect of shikonin on experimental colitis induced by dextran sulfate sodium in BALB/c mice.Evidence-based complementary and alternative medicine : eCAM · 2012Article
- Ewing's Sarcoma: Development of RNA Interference-Based Therapy for Advanced Disease.ISRN oncology · 2012Article
- Downregulation of STAT3 signaling induces apoptosis but also promotes anti-apoptotic gene expression in human pancreatic cancer cell lines.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2011Article
- A STAT3-decoy oligonucleotide induces cell death in a human colorectal carcinoma cell line by blocking nuclear transfer of STAT3 and STAT3-bound NF-κB.BMC cell biology · 2011Article
- Significance of Bcl-xL in human colon carcinoma.World journal of gastroenterology · 2008Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo investigate the roles and mechanism of signal transducer and activator of transcription 3 (STAT3) in invasion of human colon cancer cells by RNA interference.
methodsSmall interfering RNA (siRNA) targeting Signal transducer and activator of transcription 3 (STAT3) was transfected into HT29 colon cancer cells. STAT3 protein level and DNA-binding activity of STAT3 was evaluated by western blotting and electrophoretic mobility shift assay (EMSA), respectively. We studied the anchorage-independent growth using colony formation in soft agar, and invasion using the boyden chamber model, anoikis using DNA fragmentation assay and terminal deoxynucleotidyltransferase-mediated dUTP nick-end labeling (TUNEL), respectively. Western blot assay was used to observe the protein expression of Bcl-xL and survivin in colon cancer HT29 cells.
resultsRNA interference (RNAi) mediated by siRNA leads to suppression of STAT3 expression in colon cancer cell lines. Suppression of STAT3 expression by siRNA could inhibit anchorage-independent growth, and invasion ability, and induces anoikis in the colon cancer cell line HT29. It has been shown that knockdown of STAT3 expression by siRNA results in a reduction in expression of Bcl-xL and survivin in HT29 cells.
conclusionThese results suggest that STAT3 siRNA can inhibit the invasion ability of colon cancer cells through inducing anoikis, which antiapoptotic genes survivin and Bcl-xL contribute to regulation of anoikis. These studies indicate STAT3 siRNA could be a useful therapeutic tool for the treatment of colon cancer.
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