ArticleChromosoma2008
Size and number of tandem repeat arrays can determine somatic homologous pairing of transgene loci mediated by epigenetic modifications in Arabidopsis thaliana nuclei.
Article in Chromosoma, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- Live cell imaging of DNA and RNA with fluorescent signal amplification and background reduction techniques.Frontiers in cell and developmental biology · 2023Review
- Application and prospects of CRISPR/Cas9-based methods to trace defined genomic sequences in living and fixed plant cells.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2020Review
- The arrangement of Brachypodium distachyon chromosomes in interphase nuclei.Journal of experimental botany · 2016Article
- Stable gene replacement in barley by targeted double-strand break induction.Journal of experimental botany · 2016Article
- Chromatin Ring Formation at Plant Centromeres.Frontiers in plant science · 2016Article
- DNA double-strand breaks alter the spatial arrangement of homologous loci in plant cells.Scientific reports · 2015Article
- Engineering of plant chromosomes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Review
- Protein-mediated chromosome pairing of repetitive arrays.Journal of molecular biology · 2014Article
- Decoding the role of chromatin architecture in development: coming closer to the end of the tunnel.Frontiers in plant science · 2014Review
- The Arabidopsis CAP-D proteins are required for correct chromatin organisation, growth and fertility.Chromosoma · 2013Article
- De novo generation of plant centromeres at tandem repeats.Chromosoma · 2013Article
- Interphase chromatin organisation in Arabidopsis nuclei: constraints versus randomness.Chromosoma · 2012Article
- Article
- High frequency, cell type-specific visualization of fluorescent-tagged genomic sites in interphase and mitotic cells of living Arabidopsis plants.Plant methods · 2010Article
- The STRUCTURAL MAINTENANCE OF CHROMOSOMES 5/6 complex promotes sister chromatid alignment and homologous recombination after DNA damage in Arabidopsis thaliana.The Plant cell · 2009Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The chromosomal arrangement of different transgenic repeat arrays inserted at various chromosomal positions was tested by FISH in Arabidopsis 2C leaf and root nuclei. Large lacO ( approximately 10 kb) but not tetO (4.8 kb) or small lacO ( approximately 2 kb) arrays were, in general, more often spatially associated with heterochromatic chromocenters (CC) than flanking regions (that either overlap the array insert position or are between 5 and 163 kb apart from the insert site). Allelic and ectopic pairing frequencies of lacO arrays were significantly increased only in nuclei of lines with two large lacO arrays inserted at different positions on the same chromosome arm. Within the same lines, root nuclei showed a significantly lower increase of pairing frequencies at the insert position compared to leaf nuclei but still a higher frequency than in the wild-type situation. Thus, the frequencies of homologous pairing and association with heterochromatin of transgenic repeats may differ with the construct, the chromosomal insertion position, the cell type and with the number and repetitiveness of inserts. Strong CpG methylation is correlated with a high frequency of homologous pairing at large repeat array loci in somatic cells but has no impact on their association with CCs. These results show that single low-copy arrays apparently do not alter interphase chromatin architecture and are more suitable for chromatin tagging than multiple high copy arrays.
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