ArticleGene therapy2008
Gene transfer with a vector expressing Maxi-K from a smooth muscle-specific promoter restores erectile function in the aging rat.
Article in Gene therapy, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 42 citations in OpenAlex.
- Erectile Dysfunction: Treatments, Advances and New Therapeutic Strategies.Brain sciences · 2023Review
- Gene Therapy for Overactive Bladder: A Review of BK-Channel α-Subunit Gene Transfer.Therapeutics and clinical risk management · 2021Review
- Testosterone regulates the expression and functional activity of sphingosine-1-phosphate receptors in the rat corpus cavernosum.Journal of cellular and molecular medicine · 2018Article
- Topically applied NO-releasing nanoparticles can increase intracorporal pressure and elicit spontaneous erections in a rat model of radical prostatectomy.The journal of sexual medicine · 2014Article
- Large-conductance voltage- and Ca2+-activated K+ channel regulation by protein kinase C in guinea pig urinary bladder smooth muscle.American journal of physiology. Cell physiology · 2014Article
- Erectile dysfunction - an update of current practice and future strategies.Journal of clinical urology · 2013Article
- Article
- Emerging tools for erectile dysfunction: a role for regenerative medicine.Nature reviews. Urology · 2012Review
- Suppression of human detrusor smooth muscle excitability and contractility via pharmacological activation of large conductance Ca2+-activated K+ channels.American journal of physiology. Cell physiology · 2012Article
- Smooth muscle myosin expression, isoform composition, and functional activities in rat corpus cavernosum altered by the streptozotocin-induced type 1 diabetes.American journal of physiology. Endocrinology and metabolism · 2012Article
- Emerging and novel therapeutic approaches in the treatment of male erectile dysfunction.Current urology reports · 2011Review
- Large-conductance voltage- and Ca2+-activated K+ channels regulate human detrusor smooth muscle function.American journal of physiology. Cell physiology · 2011Article
- Nucleic acid therapy for lifespan prolongation: present and future.Journal of biosciences · 2011Review
- Silencing MaxiK activity in corporal smooth muscle cells initiates compensatory mechanisms to maintain calcium homeostasis.The journal of sexual medicine · 2011Article
- Gene therapy as future treatment of erectile dysfunction.Expert opinion on biological therapy · 2010Review
- The mechanism of opiorphin-induced experimental priapism in rats involves activation of the polyamine synthetic pathway.American journal of physiology. Cell physiology · 2009Article
- Vcsa1 acts as a marker of erectile function recovery after gene therapeutic and pharmacological interventions.The Journal of urology · 2009Article
- Testosterone regulates erectile function and Vcsa1 expression in the corpora of rats.Molecular and cellular endocrinology · 2009Article
- The role of opiorphins (endogenous neutral endopeptidase inhibitors) in urogenital smooth muscle biology.The journal of sexual medicine · 2009Review
- Hypercontractility and impaired sildenafil relaxations in the BKCa channel deletion model of erectile dysfunction.American journal of physiology. Regulatory, integrative and comparative physiology · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previous reports have demonstrated that gene transfer with the alpha, or pore-forming, subunit of the human Maxi-K channel (hSlo) restores the decline in erectile capacity observed in established rat models of diabetes and aging. Preliminary data from a human clinical trial also showed safety and potential efficacy in 11 men treated with the same plasmid construct expressing the Maxi-K channel. In all instances, the original plasmid was driven by the heterologous cytomegalovirus promoter which is broadly active in a wide variety of cell and tissue types. To more precisely determine the contribution of the corporal myocyte to the observed physiological effects in vivo, we report here our initial work using a distinct vector (pSMAA-hSlo) in which hSlo gene expression was driven off the mouse smooth muscle alpha-actin (SMAA) promoter. Specifically, older rats, with diminished erectile capacity, were given a single intracorporal injection with either 100 mug pVAX-hSlo or 10, 100 or 1000 mug pSMAA-hSlo, or vector or vehicle alone. Significantly increased intracavernous pressure (ICP) responses to cavernous nerve stimulation were observed for all doses of both plasmids encoding hSlo, relative to control injections. These data confirm and extend previous observations to document that smooth muscle cell-specific expression of hSlo in corporal tissue is both necessary and sufficient to restore erectile function in aging rats.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.