ArticleThe EMBO journal2008
Structure of the Eps15-stonin2 complex provides a molecular explanation for EH-domain ligand specificity.
Article in The EMBO journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
- Promiscuous and multivalent interactions between Eps15 and partner protein Dab2 generate a complex interaction network.Nature communications · 2025Article
- Tackling Undruggable Targets with Designer Peptidomimetics and Synthetic Biologics.Chemical reviews · 2024Review
- An extended interaction site determines binding between AP180 and AP2 in clathrin mediated endocytosis.Nature communications · 2024Article
- Completing the family of human Eps15 homology domains: Solution structure of the internal Eps15 homology domain of γ-synergin.Protein science : a publication of the Protein Society · 2022Article
- Distinct EH domains of the endocytic TPLATE complex confer lipid and protein binding.Nature communications · 2021Article
- A nanobody-based molecular toolkit provides new mechanistic insight into clathrin-coat initiation.eLife · 2019Article
- Intersectin scaffold proteins and their role in cell signaling and endocytosis.Biochimica et biophysica acta. Molecular cell research · 2017Review
- Structural basis for the recognition of two consecutive mutually interacting DPF motifs by the SGIP1 μ homology domain.Scientific reports · 2016Article
- Conformational Restriction of Peptides Using Dithiol Bis-Alkylation.Methods in enzymology · 2016Article
- Predicting physical interactions between protein complexes.Molecular & cellular proteomics : MCP · 2013Article
- A snapshot of the physical and functional wiring of the Eps15 homology domain network in the nematode.PloS one · 2013Article
- Secretory carrier membrane proteins.Protoplasma · 2012Review
- Article
- EHD proteins: key conductors of endocytic transport.Trends in cell biology · 2011Review
- Charge effects in the selection of NPF motifs by the EH domain of EHD1.Biochemistry · 2010Article
- ELM: the status of the 2010 eukaryotic linear motif resource.Nucleic acids research · 2010Article
- Structural insight into the interaction of proteins containing NPF, DPF, and GPF motifs with the C-terminal EH-domain of EHD1.Protein science : a publication of the Protein Society · 2009Article
- Mechanisms of EHD/RME-1 protein function in endocytic transport.Traffic (Copenhagen, Denmark) · 2008Review
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Eps15 homology (EH) domain-containing proteins play a key regulatory role in intracellular membrane trafficking and cell signalling. EH domains serve as interaction platforms for short peptide motifs comprising the residues NPF within natively unstructured regions of accessory proteins. The EH-NPF interactions described thus far are of very low affinity and specificity. Here, we identify the presynaptic endocytic sorting adaptor stonin2 as a high-affinity ligand for the second EH domain (EH2) of the clathrin accessory protein Eps15. Calorimetric data indicate that both NPF motifs within stonin2 interact with EH2 simultaneously and with sub-micromolar affinity. The solution structure of this complex reveals that the first NPF motif binds to the conserved site on the EH domain, whereas the second motif inserts into a novel hydrophobic pocket. Our data show how combination of two EH-attachment sites provides a means for modulating specificity and allows discrimination from a large pool of potential binding partners containing NPF motifs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.