ArticleBlood2008
Basal and angiopoietin-1-mediated endothelial permeability is regulated by sphingosine kinase-1.
Article in Blood, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to 2 registered trials, which are not on this map. Cited by 41 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Acute Effects of Particulate Matter on Pulmonary Diseases: Discovery Its Chemo-signatures
To Assess the Role of Sphingosine-1-phosphate in the Pathobiology of Pneumonia: Generate a New Strategy for Treatment of Severe Community-acquired Pneumonia
Who cites it
41 citing papers in PubMed, 88 citations in OpenAlex.
- Review
- Indoxyl sulfate induces retinal microvascular injury via COX-2/PGEJournal of translational medicine · 2024Article
- Barrier maintenance by S1P during inflammation and sepsis.Tissue barriers · 2021Review
- Sphingosine Kinase 1 Plays an Important Role in Atorvastatin-Mediated Anti-Inflammatory Effect against Acute Lung Injury.Mediators of inflammation · 2021Article
- Biomaterials for Bioprinting Microvasculature.Chemical reviews · 2020Review
- The Angiopoietin-Tie2 Pathway in Critical Illness.Critical care clinics · 2020Review
- Downregulation of S1P Lyase Improves Barrier Function in Human Cerebral Microvascular Endothelial Cells Following an Inflammatory Challenge.International journal of molecular sciences · 2020Article
- Novel mechanisms regulating endothelial barrier function in the pulmonary microcirculation.The Journal of physiology · 2019Review
- Circulating sphingosine-1-phosphate as a prognostic biomarker for community-acquired pneumonia.PloS one · 2019Article
- Multiplexed, high-throughput measurements of cell contraction and endothelial barrier function.Laboratory investigation; a journal of technical methods and pathology · 2019Article
- Secreted Phospholipases AFrontiers in immunology · 2018Article
- The Angiopoietin-Tie2 Signaling Axis in Systemic Inflammation.Journal of the American Society of Nephrology : JASN · 2017Review
- Vascular and Immunobiology of the Circulatory Sphingosine 1-Phosphate Gradient.Annual review of physiology · 2017Review
- Platelet and Erythrocyte Sources of S1P Are Redundant for Vascular Development and Homeostasis, but Both Rendered Essential After Plasma S1P Depletion in Anaphylactic Shock.Circulation research · 2016Article
- HDL-S1P: cardiovascular functions, disease-associated alterations, and therapeutic applications.Frontiers in pharmacology · 2015Review
- Dengue Virus-Induced Inflammation of the Endothelium and the Potential Roles of Sphingosine Kinase-1 and MicroRNAs.Mediators of inflammation · 2015Review
- The angiopoietin-Tie2 signaling axis in the vascular leakage of systemic inflammation.Tissue barriers · 2015Article
- The role of sphingosine-1-phosphate in endothelial barrier function.Biochimica et biophysica acta · 2014Review
- Dynamics of pulmonary endothelial barrier function in acute inflammation: mechanisms and therapeutic perspectives.Cell and tissue research · 2014Review
- Regulation of angiopoietin-1/Tie-2 receptor signaling in endothelial cells by dual-specificity phosphatases 1, 4, and 5.Journal of the American Heart Association · 2013Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 2 countries.
Funding
Abstract
Endothelial cells (ECs) regulate the barrier function of blood vessels. Here we show that basal and angiopoietin-1 (Ang-1)-regulated control of EC permeability is mediated by 2 different functional states of sphingosine kinase-1 (SK-1). Mice depleted of SK-1 have increased vascular leakiness, whereas mice transgenic for SK-1 in ECs show attenuation of leakiness. Furthermore, Ang-1 rapidly and transiently stimulates SK-1 activity and phosphorylation, and induces an increase in intracellular sphingosine-1-phosphate (S1P) concentration. Overexpression of SK-1 resulted in inhibition of permeability similar to that seen for Ang-1, whereas knockdown of SK-1 by small interfering RNA blocked Ang-1-mediated inhibition of permeability. Transfection with SKS225A, a nonphosphorylatable mutant of SK-1, inhibited basal leakiness, and both SKS225A and a dominant-negative SK-1 mutant removed the capacity of Ang-1 to inhibit permeability. These effects were independent of extracellular S1P as knockdown or inhibition of S1P1, S1P2, or S1P3, did not affect the Ang-1 response. Thus, SK-1 levels in ECs powerfully regulate basal permeability in vitro and in vivo. In addition, the Ang-1-induced inhibition of leakiness is mediated through activation of SK-1, defining a new signaling pathway in the Ang-1 regulation of permeability.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.