ArticleJournal of clinical microbiology2008
Evaluation of different cytomegalovirus (CMV) DNA PCR protocols for analysis of dried blood spots from consecutive cases of neonates with congenital CMV infections.
Article in Journal of clinical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 2 of them syntheses that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 2 syntheses or guidelines pooled it, 110 citations in OpenAlex.
- Detection of Viral Nucleic Acid in Specimens Spotted on Commercial Filter Papers: A Review and Meta-Analysis.Viruses · 2026Pooled it
- Dried blood spots PCR assays to screen congenital cytomegalovirus infection: a meta-analysis.Virology journal · 2015Pooled it
- A pilot study using residual newborn dried blood spots to assess the potential role of cytomegalovirus and Toxoplasma gondii in the etiology of congenital hydrocephalus.Birth defects research. Part A, Clinical and molecular teratology · 2013Trial
- Screening of congenital cytomegalovirus infection by real-time PCR in urine pools.European journal of pediatrics · 2012Trial
- Saliva polymerase-chain-reaction assay for cytomegalovirus screening in newborns.The New England journal of medicine · 2011Trial
- PCR-based detection of cytomegalovirus in neonates and children, with emphasis on congenital and neonatal-screening cohorts: A systematic review and meta-analysis.SAGE open medicine · 2026Review
- Role of TLR4 Asp299Gly and Thr399Ile Gene Polymorphisms as a Risk Factor in Human Cytomegalovirus Positive Pregnant Women.Reproductive sciences (Thousand Oaks, Calif.) · 2025Article
- Comparison of the recovery of PCR-detectable porcine epidemic diarrhea virus and porcine reproductive and respiratory syndrome virus from filter papers under laboratory conditions.Frontiers in cellular and infection microbiology · 2025Article
- Utility of Dried Blood Spots for the Diagnosis of Congenital Cytomegaloviruses within the First 21 Days of Life in a Single Center.International journal of neonatal screening · 2023Article
- Review
- Delays in diagnosis and treatment initiation for congenital cytomegalovirus infection - Why we need universal screening.Frontiers in pediatrics · 2022Article
- The frequency of cytomegalovirus non-ELR UL146 genotypes in neonates with congenital CMV disease is comparable to strains in the background population.BMC infectious diseases · 2021Article
- A Systematic Review on the Association of Acquired Human Cytomegalovirus Infection with Hearing Loss.Journal of clinical medicine · 2020Review
- Congenital Cytomegalovirus Infection: Update on Diagnosis and Treatment.Microorganisms · 2020Review
- Diagnosing congenital Cytomegalovirus infection: don't get rid of dried blood spots.BMC infectious diseases · 2020Review
- Article
- Detection of congenital cytomegalovirus in newborns using nucleic acid amplification techniques and its public health implications.Virologica Sinica · 2017Review
- Quantification of Active and Latent Form of Human Cytomegalovirus Infection in Umbilical Cord Blood Donors by Real-Time PCR.International journal of organ transplantation medicine · 2017Article
- Novel keratin 5 mutation in a family with epidermolysis bullosa simplex.Experimental and therapeutic medicine · 2015Article
- Evaluation of a new protocol for retrospective diagnosis of congenital toxoplasmosis by use of Guthrie cards.Journal of clinical microbiology · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two protocols for the extraction of cytomegalovirus (CMV) DNA and two methods for the amplification of CMV DNA in dried blood spots were evaluated for the retrospective diagnosis of congenital CMV infection. During the period from 1996 to 2006, a urine screening program detected 76 congenitally infected neonates. Stored Guthrie cards with blood from 55 cases and 12 controls were tested. Two spots of dried blood were cut from each card and evaluated in two centers. CMV DNA was extracted from a whole single spot. Center 1 used phenol-chloroform extraction and ethanol precipitation followed by a conventional PCR. Center 2 used the NucliSens easyMAG automated DNA/RNA extraction platform (bioMérieux) followed by a real-time PCR. For evaluation of the extraction method, DNA extracted from each blood spot was evaluated by the amplification method used by the collaborating center. The sensitivities were 66% for center 1 and 73% for center 2. None of the controls were positive. A sensitivity as high as 82% could be obtained by combining the most sensitive extraction method (the phenol-chloroform procedure) with the most sensitive PCR method (real-time PCR). The detection rate was not influenced by the duration of storage of the spots. The sensitivity was higher with blood from congenitally infected cases due to a primary maternal CMV infection, regardless of the protocol used. However, the difference reached significance only for the least-sensitive protocol (P = 0.036).
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