ArticleClinical and vaccine immunology : CVI2008
Rational combination of peptides derived from different Mycobacterium leprae proteins improves sensitivity for immunodiagnosis of M. leprae infection.
Article in Clinical and vaccine immunology : CVI, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 47 citations in OpenAlex.
- Immunologically reactive M. leprae antigens with relevance to diagnosis and vaccine development.BMC infectious diseases · 2011Trial
- A Multiepitope Protein Based on Selected Synthetic Peptides Enables Effective Serological Screening of Exposure toACS omega · 2026Article
- Longitudinal immune profiles in type 1 leprosy reactions in Bangladesh, Brazil, Ethiopia and Nepal.BMC infectious diseases · 2015Article
- Successive Intramuscular Boosting with IFN-Alpha Protects Mycobacterium bovis BCG-Vaccinated Mice against M. lepraemurium Infection.BioMed research international · 2015Article
- T-cell regulation in lepromatous leprosy.PLoS neglected tropical diseases · 2014Article
- Phage display and synthetic peptides as promising biotechnological tools for the serological diagnosis of leprosy.PloS one · 2014Article
- Analysis of antigens of Mycobacterium leprae by interaction to sera IgG, IgM, and IgA response to improve diagnosis of leprosy.BioMed research international · 2014Review
- Gene expression profile and immunological evaluation of unique hypothetical unknown proteins of Mycobacterium leprae by using quantitative real-time PCR.Clinical and vaccine immunology : CVI · 2013Article
- Identification of mimotopes of Mycobacterium leprae as potential diagnostic reagents.BMC infectious diseases · 2013Article
- Impairment of IFN-gamma response to synthetic peptides of Mycobacterium tuberculosis in a 7-day whole blood assay.PloS one · 2013Article
- Evaluation of various cytokines elicited during antigen-specific recall as potential risk indicators for the differential development of leprosy.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2012Article
- New biomarkers with relevance to leprosy diagnosis applicable in areas hyperendemic for leprosy.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Pathogen-specific epitopes as epidemiological tools for defining the magnitude of Mycobacterium leprae transmission in areas endemic for leprosy.PLoS neglected tropical diseases · 2012Article
- Peptides Derived from Mycobacterium leprae ML1601c Discriminate between Leprosy Patients and Healthy Endemic Controls.Journal of tropical medicine · 2012Article
- ML1419c peptide immunization induces Mycobacterium leprae-specific HLA-A*0201-restricted CTL in vivo with potential to kill live mycobacteria.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- Mycobacterium leprae-host-cell interactions and genetic determinants in leprosy: an overview.Future microbiology · 2011Review
- Enhancing sensitivity of detection of immune responses to Mycobacterium leprae peptides in whole-blood assays.Clinical and vaccine immunology : CVI · 2010Article
- From genome-based in silico predictions to ex vivo verification of leprosy diagnosis.Clinical and vaccine immunology : CVI · 2009Article
- Antigen-specific T-cell responses of leprosy patients.Clinical and vaccine immunology : CVI · 2008Article
- ML Flow serological test: complementary tool in leprosy.Anais brasileiros de dermatologiaArticle
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The stable incidence of new leprosy cases suggests that transmission of infection is continuing despite the worldwide implementation of multidrug therapy programs. Highly specific tools are required to accurately diagnose asymptomatic and early stage Mycobacterium leprae infections which are the likely sources of transmission and cannot be identified by using the detection of antibodies against phenolic glycolipid I. One of the hurdles hampering T-cell-based diagnostic tests is that M. leprae antigens cross-react at the T-cell level with antigens present in other mycobacteria, like M. tuberculosis or M. bovis bacillus Calmette-Guerin (BCG). Using comparative genomics, we previously identified five candidate proteins highly restricted to M. leprae which showed promising features with respect to application in leprosy diagnostics. However, despite the lack of overall sequence homology, the use of recombinant proteins includes the risk of detecting T-cell responses that are cross-reactive with other antigens. To improve the diagnostic potential of these M. leprae sequences, we used 50 synthetic peptides spanning the sequences of all five proteins for the induction of T-cell responses (gamma interferon) in leprosy patients, healthy household contacts (HHC) of leprosy patients, and healthy controls in Brazil, as well as in tuberculosis patients, BCG vaccinees, and healthy subjects from an area of nonendemicity. Using the combined T-cell responses toward four of these peptides, all paucibacillary patients and 13 out of 14 HHC were detected without compromising specificity. The peptides contain HLA binding motifs for various HLA class I and II alleles, thereby meeting an important requirement for the applicability of diagnostic tools in genetically diverse populations. Thus, this study provides the first evidence for the possibility of immunodiagnostics for leprosy based on mixtures of peptides recognized in the context of different HLA alleles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.