ArticleAmerican journal of physiology. Cell physiology2008
Two-dimensional kinetics of beta 2-integrin and ICAM-1 bindings between neutrophils and melanoma cells in a shear flow.
Article in American journal of physiology. Cell physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 43 citations in OpenAlex.
- Melanoma cell adhesion molecule (MCAM) mediates microtentacle generation, homotypic clustering, and reattachment of non-adherent breast tumor cells.Breast cancer research : BCR · 2026Article
- Circulating Tumor Cells for the Monitoring of Lung Cancer Therapies.International journal of molecular sciences · 2025Review
- Polymorphonuclear Neutrophils and Tumors: Friend or Foe?Experientia supplementum (2012) · 2022Article
- A narrative review of circulating tumor cells clusters: A key morphology of cancer cells in circulation promote hematogenous metastasis.Frontiers in oncology · 2022Review
- Fluid shear stress and tumor metastasis.American journal of cancer research · 2018Review
- Neutrophil Extracellular Trap Production in Patients with Colorectal Cancer In Vitro.International journal of inflammation · 2017Article
- Pivotal role of pervasive neoplastic and stromal cells reprogramming in circulating tumor cells dissemination and metastatic colonization.Cancer microenvironment : official journal of the International Cancer Microenvironment Society · 2014Article
- Neutrophil extracellular traps in cancer progression.Cellular and molecular life sciences : CMLS · 2014Review
- Melanoma upregulates ICAM-1 expression on endothelial cells through engagement of tumor CD44 with endothelial E-selectin and activation of a PKCα-p38-SP-1 pathway.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2014Article
- Mechanomics: an emerging field between biology and biomechanics.Protein & cell · 2014Review
- Neutrophil extracellular traps sequester circulating tumor cells and promote metastasis.The Journal of clinical investigation · 2013Article
- LFA-1 and ICAM-1 expression induced during melanoma-endothelial cell co-culture favors the transendothelial migration of melanoma cell lines in vitro.BMC cancer · 2012Article
- Fibrin serves as a divalent ligand that regulates neutrophil-mediated melanoma cells adhesion to endothelium under shear conditions.American journal of physiology. Cell physiology · 2012Article
- Adhesion receptors as therapeutic targets for circulating tumor cells.Frontiers in oncology · 2012Article
- Determining beta2-integrin and intercellular adhesion molecule 1 binding kinetics in tumor cell adhesion to leukocytes and endothelial cells by a gas-driven micropipette assay.The Journal of biological chemistry · 2011Article
- Sequential binding of αVβ3 and ICAM-1 determines fibrin-mediated melanoma capture and stable adhesion to CD11b/CD18 on neutrophils.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- Transiently entrapped circulating tumor cells interact with neutrophils to facilitate lung metastasis development.Cancer research · 2010Article
- Tumor cell extravasation mediated by leukocyte adhesion is shear rate dependent on IL-8 signaling.Molecular & cellular biomechanics : MCB · 2010Article
- Application of Population Dynamics to Study Heterotypic Cell Aggregations in the Near-Wall Region of a Shear Flow.Cellular and molecular bioengineering · 2010Article
- Immunoediting of leukocyte functions within the tumor microenvironment promotes cancer metastasis development.Biorheology · 2009Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Cell adhesion, mediated by specific receptor-ligand interactions, plays an important role in biological processes such as tumor metastasis and inflammatory cascade. For example, interactions between beta 2-integrin (lymphocyte function-associated antigen-1 and/or Mac-1) on polymorphonuclear neutrophils (PMNs) and ICAM-1 on melanoma cells initiate the bindings of melanoma cells to PMNs within the tumor microenvironment in blood flow, which in turn activate PMN-melanoma cell aggregation in a near-wall region of the vascular endothelium, therefore enhancing subsequent extravasation of melanoma cells in the microcirculations. Kinetics of integrin-ligand bindings in a shear flow is the determinant of such a process, which has not been well understood. In the present study, interactions of PMNs with WM9 melanoma cells were investigated to quantify the kinetics of beta 2-integrin and ICAM-1 bindings using a cone-plate viscometer that generates a linear shear flow combined with a two-color flow cytometry technique. Aggregation fractions exhibited a transition phase where it first increased before 60 s and then decreased with shear durations. Melanoma-PMN aggregation was also found to be inversely correlated with the shear rate. A previously developed probabilistic model was modified to predict the time dependence of aggregation fractions at different shear rates and medium viscosities. Kinetic parameters of beta 2-integrin and ICAM-1 bindings were obtained by individual or global fittings, which were comparable to respectively published values. These findings provide new quantitative understanding of the biophysical basis of leukocyte-tumor cell interactions mediated by specific receptor-ligand interactions under shear flow conditions.
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