ArticleBiophysical journal2008
Imaging single virus particles on the surface of cell membranes by high-resolution scanning surface confocal microscopy.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Applications of Nanopipettes in Scanning Ion Conductance Microscopy for High-Spatial-Resolution Topographic Imaging and Sensing in Single Cells.ACS measurement science au · 2026Review
- Scanning Ion Conductance Microscopy.Chemical reviews · 2021Review
- Correlative 3D microscopy of single cells using super-resolution and scanning ion-conductance microscopy.Nature communications · 2021Article
- Scanning Ion Conductance Microscopy Study Reveals the Disruption of the Integrity of the Human Cell Membrane Structure by Oxidative DNA Damage.ACS applied bio materials · 2021Article
- Rapid formation of human immunodeficiency virus-like particles.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Review
- Bioinspired Protein Channel-Based Scanning Ion Conductance Microscopy (Bio-SICM) for Simultaneous Conductance and Specific Molecular Imaging.Journal of the American Chemical Society · 2016Article
- Wide-field optical detection of nanoparticles using on-chip microscopy and self-assembled nanolenses.Nature photonics · 2013Article
- Imaging the cell surface and its organization down to the level of single molecules.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Review
- Enumerating virus-like particles in an optically concentrated suspension by fluorescence correlation spectroscopy.Biomedical optics express · 2013Article
- Scanning ion conductance microscopy for studying biological samples.Sensors (Basel, Switzerland) · 2012Review
- Topographical and electrochemical nanoscale imaging of living cells using voltage-switching mode scanning electrochemical microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Scanning ion conductance microscopy: a nanotechnology for biological studies in live cells.Frontiers in physiology · 2012Article
- Scanning ion conductance microscopy: a convergent high-resolution technology for multi-parametric analysis of living cardiovascular cells.Journal of the Royal Society, Interface · 2011Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
We have developed a high-resolution scanning surface confocal microscopy technique capable of imaging single virus-like particles (VLPs) on the surfaces of cells topographically and by fluorescence. The technique combines recently published single-molecule-resolution ion-conductance microscopy that acquires topographical data with confocal microscopy providing simultaneous fluorescent imaging. In our experiments we have demonstrated that the cell membrane exhibits numerous submicrometer-sized surface structures that could be topographically confused with virus particles. However, simultaneous acquisition of confocal images allows the positions of fluorescently tagged particles to be identified. Using this technique, we have, for the first time, visualized single polyoma VLPs adsorbed onto the cell membrane. Observed VLPs had a mean width of 108 +/- 16 nm. The particles were randomly distributed across the cell membrane, and no specific interactions were seen with cell membrane structures such as microvilli. These experiments demonstrate the utility of this new microscope for imaging the interactions of nanoparticles with the cell surface to provide novel insights into the earliest interactions of viruses and other nanoparticles such as gene therapy vectors with the cell.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.