ArticleJournal of virology2008
Expression of transgenes from newcastle disease virus with a segmented genome.
Article in Journal of virology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 31 citations in OpenAlex.
- Bovine Adenovirus 3-Based Viral Vectors for Veterinary Vaccine Development: Progress, Limitations, and Future Directions.Veterinary sciences · 2026Review
- Developments in Negative-Strand RNA Virus Reverse Genetics.Microorganisms · 2024Review
- Engineering Non-Human RNA Viruses for Cancer Therapy.Vaccines · 2023Review
- Development and Scalable Production of Newcastle Disease Virus-Vectored Vaccines for Human and Veterinary Use.Viruses · 2022Review
- A Newcastle disease virus expressing a stabilized spike protein of SARS-CoV-2 induces protective immune responses.Nature communications · 2021Article
- Newcastle Disease Virus as a Vaccine Vector for 20 Years: A Focus on Maternally Derived Antibody Interference.Vaccines · 2020Review
- Newcastle disease vaccines-A solved problem or a continuous challenge?Veterinary microbiology · 2017Review
- Reverse Genetics of Newcastle Disease Virus.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Peste des petits ruminants.Veterinary microbiology · 2015Review
- Inhibition of Pseudomonas aeruginosa with a recombinant RNA-based viral vector expressing human β-defensin 4.BMC microbiology · 2014Article
- A majority of infectious Newcastle disease virus particles contain a single genome, while a minority contain multiple genomes.Journal of virology · 2012Article
- The influenza virus protein PB1-F2 inhibits the induction of type I interferon at the level of the MAVS adaptor protein.PLoS pathogens · 2011Article
- Detection of free peritoneal cancer cells in gastric cancer using cancer-specific Newcastle disease virus.Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract · 2010Article
- Glycoprotein interactions in paramyxovirus fusion.Future virology · 2009Article
- Optimization of human immunodeficiency virus gag expression by newcastle disease virus vectors for the induction of potent immune responses.Journal of virology · 2009Article
- Recombinant vectors as influenza vaccines.Current topics in microbiology and immunology · 2009Review
- Engineered intermonomeric disulfide bonds in the globular domain of Newcastle disease virus hemagglutinin-neuraminidase protein: implications for the mechanism of fusion promotion.Journal of virology · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Paramyxoviruses belong to the Paramyxoviridae family of the order Mononegavirales. They have a nonsegmented negative-stranded RNA genome and can cause a number of diseases in humans and animals. We generated a recombinant Newcastle disease virus (NDV) possessing a two-segmented genome. Each genomic segment is flanked by authentic NDV 3' and 5' noncoding termini allowing for efficient replication and transcription. A reporter gene encoding green fluorescent protein (GFP) was inserted into one segment, and a red fluorescent protein dsRed gene was inserted into the other segment in order to easily detect the replication and transcription of segments in infected cells. The rescued viruses grew well and were stable in embryonated chicken eggs over multiple passages. We were able to detect the expression of both reporter genes in the same cell infected with the virus possessing a segmented genome, and viral particles can contain either one or two types of RNA segments. We also rescued a two-segmented virus expressing GFP and the severe acute respiratory syndrome-associated coronavirus spike S protein, which is about 200 kDa. The chimeric virus extends the coding capacity of NDV by 30%, suggesting that the two-segmented NDV can be used for development of vaccines or gene therapy vectors carrying long and multiple transgenes.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.