ArticleCellular and molecular neurobiology2008
Stress upregulates TPH1 but not TPH2 mRNA in the rat dorsal raphe nucleus: identification of two TPH2 mRNA splice variants.
Article in Cellular and molecular neurobiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 57 citations in OpenAlex.
- Genetic variation in the serotonin pathway and smoking cessation with nicotine replacement therapy: new data from the Patch in Practice trial and pooled analyses.Drug and alcohol dependence · 2008Trial
- Single Intraperitoneal Injection of Dexamethasone Alerts Region-Specific Neurotransmitter Metabolism in Rat Brain.Neurochemical research · 2026Article
- Dysregulated Plasticity in Serotonin, Galanin, and Opioid Systems Contributes to Limbic Seizure Recruitment in Wistar Audiogenic Rat.Journal of neurochemistry · 2026Article
- Alterations in tryptophan metabolism andFrontiers in medicine · 2024Article
- The Impact of Depression on Detrimental Changes in Bone Microstructure in Female Mice.Neuropsychiatric disease and treatment · 2024Article
- Paternal indifference and neglect in early life and creativity: Exploring the moderating role of TPH1 genotype and offspring gender.PloS one · 2020Article
- Expression of components of the serotonergic system in the developing rat thymus.Doklady. Biochemistry and biophysics · 2017Article
- Generation of tryptophan hydroxylase 2 gene knockout pigs by CRISPR/Cas9-mediated gene targeting.Journal of biomedical research · 2017Article
- Effect of childhood general traumas on suicide attempt depends on TPH2 and ADARB1 variants in psychiatric patients.Journal of neural transmission (Vienna, Austria : 1996) · 2017Article
- Sensitization of restraint-induced corticosterone secretion after chronic restraint in rats: involvement of 5-HT₇ receptors.Neuropharmacology · 2013Article
- Endocannabinoids mediate the glucocorticoid-induced inhibition of excitatory synaptic transmission to dorsal raphe serotonin neurons.The Journal of physiology · 2012Article
- Targeting brain serotonin synthesis: insights into neurodevelopmental disorders with long-term outcomes related to negative emotionality, aggression and antisocial behaviour.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2012Review
- Chronic non-invasive corticosterone administration abolishes the diurnal pattern of tph2 expression.Psychoneuroendocrinology · 2012Article
- Serotonin and blood pressure regulation.Pharmacological reviews · 2012Review
- Advances in tryptophan hydroxylase-2 gene expression regulation: new insights into serotonin-stress interaction and clinical implications.American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics · 2012Review
- Interaction between tryptophan hydroxylase I polymorphisms and childhood abuse is associated with increased risk for borderline personality disorder in adulthood.Psychiatric genetics · 2012Article
- Development by environment interactions controlling tryptophan hydroxylase expression.Journal of chemical neuroanatomy · 2011Review
- Stress-hyperresponsive WKY rats demonstrate depressed dorsal raphe neuronal excitability and dysregulated CRF-mediated responses.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2011Article
- A genetically defined morphologically and functionally unique subset of 5-HT neurons in the mouse raphe nuclei.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Activity-dependent regulation of MHC class I expression in the developing primary visual cortex of the common marmoset monkey.Behavioral and brain functions : BBF · 2011Article
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Serotonin is implicated in stress-related psychopathologies. Two isoforms of the rate-limiting enzyme of serotonin biosynthesis, tryptophan hydroxylase, TPH1 and TPH2, are known. We show here that in the rat dorsal raphe nucleus (DRN), the nucleus that contains the highest number of 5-HT neurons in the brain, TPH1 mRNA reveals a low level of expression but is detectable both by quantitative real-time PCR and in situ hybridization whereas in the pineal gland (PiG), TPH1 mRNA is strongly expressed. To examine effects of stress on TPH expression we exposed male Wistar rats to daily restraint stress for 1 week. As shown by quantitative real-time PCR, TPH1 mRNA is 2.5-fold upregulated by the stress in DRN but not in PiG. Using 3'-RACE, we identified two TPH2 mRNA splice variants in the rat DRN which differ in the length of their 3'-untranslated regions (UTRs). TPH2b (with a short 3'-UTR) is the predominant variant in the DRN, whereas TPH2a (with a longer 3'-UTR) shows a low abundance in this nucleus. In the PiG, only TPH2b is detectable revealing a low level of expression. Expression of both TPH2 splice variants is not affected by stress, neither in DRN nor in the PiG. These data indicate that TPH1 in the serotonergic neurons of the DRN might be relevant for stress-induced psychopathologies.
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