ArticlePlant molecular biology2008
Expressed sequence tag analysis and development of gene associated markers in a near-isogenic plant system of Eragrostis curvula.
Article in Plant molecular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- A Precise Reproductive Calendar of Sexual and Apomictic Genotypes ofPlants (Basel, Switzerland) · 2026Article
- Integrative gene expression and heterologous functional analysis identify candidate regulators of apomixis inFrontiers in plant science · 2026Article
- From tetraploid to diploid, a pangenomic approach to identify genes lost during synthetic diploidization ofFrontiers in plant science · 2023Article
- A combined transcriptome - miRNAome approach revealed that aFrontiers in plant science · 2022Article
- Review
- Differential Methylation Patterns in Apomictic vs. Sexual Genotypes of the Diplosporous GrassPlants (Basel, Switzerland) · 2021Article
- Genetic Transformation of Apomictic Grasses: Progress and Constraints.Frontiers in plant science · 2021Review
- Article
- Article
- Characterization and discovery of miRNA and miRNA targets from apomictic and sexual genotypes of Eragrostis curvula.BMC genomics · 2019Article
- A high-quality genome of Eragrostis curvula grass provides insights into Poaceae evolution and supports new strategies to enhance forage quality.Scientific reports · 2019Article
- A High-Density Linkage Map of the Forage GrassFrontiers in plant science · 2019Article
- The MAP3K-CodingFrontiers in plant science · 2018Article
- De novo transcriptome sequencing and assembly from apomictic and sexual Eragrostis curvula genotypes.PloS one · 2017Article
- Increased apomixis expression concurrent with genetic and epigenetic variation in a newly synthesized Eragrostis curvula polyploid.Scientific reports · 2014Article
- Characterization of retrotransposon sequences expressed in inflorescences of apomictic and sexual Paspalum notatum plants.Sexual plant reproduction · 2011Article
- Conserved and divergent rhythms of crassulacean acid metabolism-related and core clock gene expression in the cactus Opuntia ficus-indica.Plant physiology · 2011Article
- Variation in cytosine methylation patterns during ploidy level conversions in Eragrostis curvula.Plant molecular biology · 2009Article
- Gene expression in diplosporous and sexual Eragrostis curvula genotypes with differing ploidy levels.Plant molecular biology · 2008Article
Corrections and comments
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Eragrostis curvula (Schrad.) Nees is a forage grass native to the semiarid regions of Southern Africa, which reproduces mainly by pseudogamous diplosporous apomixis. A collection of ESTs was generated from four cDNA libraries, three of them obtained from panicles of near-isogenic lines with different ploidy levels and reproductive modes, and one obtained from 12 days-old plant leaves. A total of 12,295 high-quality ESTs were clustered and assembled, rendering 8,864 unigenes, including 1,490 contigs and 7,394 singletons, with a genome coverage of 22%. A total of 7,029 (79.11%) unigenes were functionally categorized by BLASTX analysis against sequences deposited in public databases, but only 37.80% could be classified according to Gene Ontology. Sequence comparison against the cereals genes indexes (GI) revealed 50% significant hits. A total of 254 EST-SSRs were detected from 219 singletons and 35 from contigs. Di- and tri- motifs were similarly represented with percentages of 38.95 and 40.16%, respectively. In addition, 190 SNPs and Indels were detected in 18 contigs generated from 3 to 4 libraries. The ESTs and the molecular markers obtained in this study will provide valuable resources for a wide range of applications including gene identification, genetic mapping, cultivar identification, analysis of genetic diversity, phenotype mapping and marker assisted selection.
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