ArticleThe Journal of general physiology2008
Temperature dependence of fusion kinetics and fusion pores in Ca2+-triggered exocytosis from PC12 cells.
Article in The Journal of general physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed.
- Turbocharging synaptic transmission: 12 SNAREpins are required for rapid release of reconstituted synaptic vesicles.Science advances · 2026Article
- Exosome secretion kinetics are controlled by temperature.Biophysical journal · 2023Article
- How to Build a Fast and Highly Sensitive Sound Detector That Remains Robust to Temperature Shifts.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- SNARE machinery is optimized for ultrafast fusion.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Visualization of expanding fusion pores in secretory cells.The Journal of general physiology · 2018Review
- The actin binding protein scinderin acts in PC12 cells to tether dense-core vesicles prior to secretion.Molecular and cellular neurosciences · 2017Article
- Entropic forces drive self-organization and membrane fusion by SNARE proteins.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Fusion pores and their control of neurotransmitter and hormone release.The Journal of general physiology · 2017Review
- Cooling reduces cAMP-stimulated exocytosis and adiponectin secretion at a Ca2+-dependent step in 3T3-L1 adipocytes.PloS one · 2015Article
- Article
- cAMP-mediated stabilization of fusion pores in cultured rat pituitary lactotrophs.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Kv2.1 cell surface clusters are insertion platforms for ion channel delivery to the plasma membrane.Molecular biology of the cell · 2012Article
- Release mode of large and small dense-core vesicles specified by different synaptotagmin isoforms in PC12 cells.Molecular biology of the cell · 2011Article
- Regulation of exocytosis and fusion pores by synaptotagmin-effector interactions.Molecular biology of the cell · 2010Article
- Membrane bending energy and fusion pore kinetics in Ca(2+)-triggered exocytosis.Biophysical journal · 2010Article
- A fast, single-vesicle fusion assay mimics physiological SNARE requirements.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Phosphatidylserine regulation of Ca2+-triggered exocytosis and fusion pores in PC12 cells.Molecular biology of the cell · 2009Article
- Article
- Kiss-and-run exocytosis and fusion pores of secretory vesicles in human beta-cells.Pflugers Archiv : European journal of physiology · 2009Article
- Synaptic vesicle endocytosis: fast and slow modes of membrane retrieval.Trends in neurosciences · 2008Review
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
The temperature dependence of Ca(2+)-triggered exocytosis was studied using carbon fiber amperometry to record the release of norepinephrine from PC12 cells. Single-vesicle fusion events were examined at temperatures varying from 12 to 28 degrees C, and with release elicited by depolarization. Measurements were made of the initial and maximum frequencies of exocytotic events, of fusion pore lifetime, flux through the open fusion pore, kiss-and-run versus full-fusion probability, and parameters associated with the shapes of amperometric spikes. The fusion pore open-state flux, and all parameters associated with spike shape, including area, rise time, and decay time, had weak temperature dependences and activation energies in the range expected for bulk diffusion in an aqueous solution. Kiss-and-run events also varied with temperature, with lower temperatures increasing the relative probability of kiss-and-run events by approximately 50%. By contrast, kinetic parameters relating to the frequency of exocytotic events and fusion pore transitions depended much more strongly on temperature, suggesting that these processes entail structural rearrangements of proteins or lipids or both. The weak temperature dependence of spike shape suggests that after the fusion pore has started to expand, structural transitions of membrane components are no longer kinetically limiting. This indicates that the content of a vesicle is expelled completely after fusion pore expansion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.