ArticleThe Journal of cell biology2008
Regulated motion of glycoproteins revealed by direct visualization of a single cargo in the endoplasmic reticulum.
Article in The Journal of cell biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 26 citations in OpenAlex.
- Dancing with the Stars: Using Image Analysis to Study the Choreography of the Endoplasmic Reticulum and Its Partners and of Movement Within Its Tubules.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Intertwined and Finely Balanced: Endoplasmic Reticulum Morphology, Dynamics, Function, and Diseases.Cells · 2021Review
- Gelsolin dysfunction causes photoreceptor loss in induced pluripotent cell and animal retinitis pigmentosa models.Nature communications · 2017Article
- Plasmolysis-deplasmolysis causes changes in endoplasmic reticulum form, movement, flow, and cytoskeletal association.Journal of experimental botany · 2017Article
- Direct PIPNature communications · 2017Article
- Oocyte-triggered dimerization of sperm IZUMO1 promotes sperm-egg fusion in mice.Nature communications · 2015Article
- Probing endoplasmic reticulum dynamics using fluorescence imaging and photobleaching techniques.Current protocols in cell biology · 2013Article
- Palmitoylated calnexin is a key component of the ribosome-translocon complex.The EMBO journal · 2012Article
- Article
- Protein mobilities and P-selectin storage in Weibel-Palade bodies.Journal of cell science · 2010Article
- Anomalous diffusion reports on the interaction of misfolded proteins with the quality control machinery in the endoplasmic reticulum.Biophysical journal · 2010Article
- BiP availability distinguishes states of homeostasis and stress in the endoplasmic reticulum of living cells.Molecular biology of the cell · 2010Article
- Lectin chaperones help direct the maturation of glycoproteins in the endoplasmic reticulum.Biochimica et biophysica acta · 2010Review
- Structural characteristics of the plasmid-encoded toxin from enteroaggregative Escherichia coli.Biochemistry · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The quality of cargo proteins in the endoplasmic reticulum (ER) is affected by their motion during folding. To understand how the diffusion of secretory cargo proteins is regulated in the ER, we directly analyze the motion of a single cargo molecule using fluorescence imaging/fluctuation analyses. We find that the addition of two N-glycans onto the cargo dramatically alters their diffusion by transient binding to membrane components that are confined by hyperosmolarity. Via simultaneous observation of a single cargo and ER exit sites (ERESs), we could exclude ERESs as the binding sites. Remarkably, actin cytoskeleton was required for the transient binding. These results provide a molecular basis for hypertonicity-induced immobilization of cargo, which is dependent on glycosylation at multiple sites but not the completion of proper folding. We propose that diffusion of secretory glycoproteins in the ER lumen is controlled from the cytoplasm to reduce the chances of aggregation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.