ArticleMolecular and cellular biology2008
The Rpb4 subunit of RNA polymerase II contributes to cotranscriptional recruitment of 3' processing factors.
Article in Molecular and cellular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 80 citations in OpenAlex.
- Spt5's central KOW domains and the Pol II stalk collaborate to regulate chromatin and 3'-end processing in Saccharomyces cerevisiae.G3 (Bethesda, Md.) · 2026Article
- Nuclear mRNA export.Acta biochimica et biophysica Sinica · 2024Review
- Structure of the recombinant RNA polymerase from African Swine Fever Virus.Nature communications · 2024Article
- Never a dull enzyme, RNA polymerase II.Transcription · 2023Review
- Article
- The Association of Rpb4 with RNA Polymerase II Depends on CTD Ser5P Phosphatase Rtr1 and Influences mRNA Decay inInternational journal of molecular sciences · 2022Article
- Regulation of Eukaryotic RNAPs Activities by Phosphorylation.Frontiers in molecular biosciences · 2021Review
- RNA polymerase II phosphorylation and gene looping: new roles for the Rpb4/7 heterodimer in regulating gene expression.Current genetics · 2020Review
- The yeast exoribonuclease Xrn1 and associated factors modulate RNA polymerase II processivity in 5' and 3' gene regions.The Journal of biological chemistry · 2020Article
- RNA polymerase II plays an active role in the formation of gene loops through the Rpb4 subunit.Nucleic acids research · 2019Article
- Biochemical methods to characterize RNA polymerase II elongation complexes.Methods (San Diego, Calif.) · 2019Article
- Mechanistic Dissection of RNA-Binding Proteins in Regulated Gene Expression at Chromatin Levels.Cold Spring Harbor symposia on quantitative biology · 2019Article
- Tunable Expression Systems for Orthogonal DNA Replication.ACS synthetic biology · 2018Article
- Article
- Sub1 and RNAPII, until termination does them part.Transcription · 2018Review
- Sub1/PC4, a multifaceted factor: from transcription to genome stability.Current genetics · 2017Review
- The structure of transcription termination factor Nrd1 reveals an original mode for GUAA recognition.Nucleic acids research · 2017Article
- The interplay between transcription and mRNA degradation inMicrobial cell (Graz, Austria) · 2017Review
- Sub1 contacts the RNA polymerase II stalk to modulate mRNA synthesis.Nucleic acids research · 2017Article
- Antagonistic roles for the ubiquitin ligase Asr1 and the ubiquitin-specific protease Ubp3 in subtelomeric gene silencing.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The RNA polymerase II enzyme from the yeast Saccharomyces cerevisiae is a complex of 12 subunits, Rpb1 to Rpb12. Crystal structures of the full complex show that the polymerase consists of two separable components, a 10-subunit core including the catalytic active site and a heterodimer of the Rpb4 and Rpb7 subunits. To characterize the role of the Rpb4/7 heterodimer during transcription in vivo, chromatin immunoprecipitation was used to examine an rpb4Delta strain for effects on the behavior of the core polymerase as well as recruitment of other protein factors involved in transcription. Rpb4/7 cross-links throughout transcribed regions. Loss of Rpb4 results in a reduction of RNA polymerase II levels near 3' ends of multiple mRNA genes as well as a decreased association of 3'-end processing factors. Furthermore, loss of Rpb4 results in altered polyadenylation site usage at the RNA14 gene. Together, these results indicate that Rpb4 contributes to proper cotranscriptional 3'-end processing in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.