ArticleInfection and immunity2008
Critical involvement of pneumolysin in production of interleukin-1alpha and caspase-1-dependent cytokines in infection with Streptococcus pneumoniae in vitro: a novel function of pneumolysin in caspase-1 activation.
Article in Infection and immunity, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 77 citations in OpenAlex.
- TLR-mediated inflammatory responses to Streptococcus pneumoniae are highly dependent on surface expression of bacterial lipoproteins.Journal of immunology (Baltimore, Md. : 1950) · 2014Trial
- The Yin and Yang of Pneumolysin During Pneumococcal Infection.Frontiers in immunology · 2022Review
- A Novel Antimicrobial Peptide SparamosinFrontiers in microbiology · 2021Article
- Article
- ATP Facilitates Staphylococcal Enterotoxin O Induced Neutrophil IL-1β SecretionFrontiers in immunology · 2021Article
- Interaction of Macrophages and Cholesterol-Dependent Cytolysins: The Impact on Immune Response and Cellular Survival.Toxins · 2020Review
- The TLR4-MyD88 Signaling Axis Regulates Lung Monocyte Differentiation Pathways in Response toFrontiers in immunology · 2020Article
- ASC and NLRP3 maintain innate immune homeostasis in the airway through an inflammasome-independent mechanism.Mucosal immunology · 2019Article
- Hydrogen peroxide release by bacteria suppresses inflammasome-dependent innate immunity.Nature communications · 2019Article
- Interleukin-1 signaling induced by Streptococcus suis serotype 2 is strain-dependent and contributes to bacterial clearance and inflammation during systemic disease in a mouse model of infection.Veterinary research · 2019Article
- Age-related differences in IL-1 signaling and capsule serotype affect persistence of Streptococcus pneumoniae colonization.PLoS pathogens · 2018Article
- Decreased NLRP3 inflammasome expression in aged lung may contribute to increased susceptibility to secondary Streptococcus pneumoniae infection.Experimental gerontology · 2018Article
- NLRP3 inflammasome activation in aged macrophages is diminished during Streptococcus pneumoniae infection.American journal of physiology. Lung cellular and molecular physiology · 2018Article
- How Inflammasomes Inform Adaptive Immunity.Journal of molecular biology · 2018Review
- TLR4 deficiency reduces pulmonary resistance to Streptococcus pneumoniae in gut microbiota-disrupted mice.PloS one · 2018Article
- Pneumolysin-Dependent Calpain Activation and Interleukin-1α Secretion in Macrophages Infected with Streptococcus pneumoniae.Infection and immunity · 2017Article
- Article
- Streptococcus pneumoniae Endopeptidase O (PepO) Elicits a Strong Innate Immune Response in Mice via TLR2 and TLR4 Signaling Pathways.Frontiers in cellular and infection microbiology · 2016Article
- Streptococcus pneumoniae induces pyroptosis through the regulation of autophagy in murine microglia.Oncotarget · 2015Article
- Peptidoglycan Branched Stem Peptides Contribute to Streptococcus pneumoniae Virulence by Inhibiting Pneumolysin Release.PLoS pathogens · 2015Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pneumolysin is a pore-forming cytolysin known as a major virulence determinant of Streptococcus pneumoniae. This protein toxin has also been shown to activate the Toll-like receptor 4 (TLR4) signaling pathway. In this study, a mutant S. pneumoniae strain deficient in pneumolysin (Deltaply) and a recombinant pneumolysin protein (rPLY) were constructed. Upon infection of macrophages in vitro, the ability to induce the production of interleukin-1alpha (IL-1alpha), IL-1beta, and IL-18 was severely impaired in the Deltaply mutant, whereas there was no marked difference in the induction of tumor necrosis factor alpha (TNF-alpha) and IL-12p40 between the wild type and the Deltaply mutant of S. pneumoniae. When macrophages were stimulated with rPLY, the production of IL-1alpha, IL-1beta, and IL-18 was strongly induced in a TLR4-dependent manner, whereas lipopolysaccharide, a canonical TLR4 agonist, hardly induced these cytokines. In contrast, lipopolysaccharide was more potent than rPLY in inducing the production of TNF-alpha, IL-6, and IL-12p40, the cytokines requiring no caspase activation. Activation of caspase-1 was observed in macrophages stimulated with rPLY but not in those stimulated with lipopolysaccharide, and the level of activation was higher in macrophages infected with wild-type S. pneumoniae than in those infected with the Deltaply mutant. These results clearly indicate that pneumolysin plays a key role in the host response to S. pneumoniae, particularly in the induction of caspase-1-dependent cytokines.
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