ArticleJournal of cellular and molecular medicine2008
Acetylcholinesterase-R increases germ cell apoptosis but enhances sperm motility.
Article in Journal of cellular and molecular medicine, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 36 citations in OpenAlex.
- Human sperm as an in vitro toxicity model: a versatile tool for assessing the risk of environmental contaminants.Archives of toxicology · 2025Article
- Acupuncture mediates the "gut-testis axis" to improve asthenozoospermia.Frontiers in endocrinology · 2025Article
- Acetylcholinesterase Inhibitors in the Treatment of Neurodegenerative Diseases and the Role of Acetylcholinesterase in their Pathogenesis.International journal of molecular sciences · 2021Review
- Characterization of the Impact of Density Gradient Centrifugation on the Profile of the Pig Sperm Transcriptome by RNA-Seq.Frontiers in veterinary science · 2021Article
- Interacting withActa pharmaceutica Sinica. B · 2020Article
- Novel association between apoptotic sperm biomarkers with seminal biochemical parameters and acetylcholinesterase activity in patients with teratozoospermia.Journal of assisted reproduction and genetics · 2019Article
- Bisphenol A Exposure and Sperm ACHE Hydroxymethylation in Men.International journal of environmental research and public health · 2019Article
- Readthrough acetylcholinesterase (AChE-R) and regulated necrosis: pharmacological targets for the regulation of ovarian functions?Cell death & disease · 2015Article
- Neuronal AChE splice variants and their non-hydrolytic functions: redefining a target of AChE inhibitors?British journal of pharmacology · 2013Review
- RACK1 promotes non-small-cell lung cancer tumorigenicity through activating sonic hedgehog signaling pathway.The Journal of biological chemistry · 2012Article
- Article
- Early appearance and possible functions of non-neuromuscular cholinesterase activities.Frontiers in molecular neuroscience · 2012Article
- Acetylcholinesterase involvement in apoptosis.Frontiers in molecular neuroscience · 2012Article
- RACK1, A multifaceted scaffolding protein: Structure and function.Cell communication and signaling : CCS · 2011Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Changes in protein subdomains through alternative splicing often modify protein-protein interactions, altering biological processes. A relevant example is that of the stress-induced up-regulation of the acetylcholinesterase (AChE-R) splice variant, a common response in various tissues. In germ cells of male transgenic TgR mice, AChE-R excess associates with reduced sperm differentiation and sperm counts. To explore the mechanism(s) by which AChE-R up-regulation affects spermatogenesis, we identified AChE-R's protein partners through a yeast two-hybrid screen. In meiotic spermatocytes from TgR mice, we detected AChE-R interaction with the scaffold protein RACK1 and elevated apoptosis. This correlated with reduced scavenging by RACK1 of the pro-apoptotic TAp73, an outcome compatible with the increased apoptosis. In contrast, at later stages in sperm development, AChE-R's interaction with the glycolytic enzyme enolase-alpha elevates enolase activity. In transfected cells, enforced AChE-R excess increased glucose uptake and adenosine tri-phosphate (ATP) levels. Correspondingly, TgR sperm cells display elevated ATP levels, mitochondrial hyperactivity and increased motility. In human donors' sperm, we found direct association of sperm motility with AChE-R expression. Interchanging interactions with RACK1 and enolase-alpha may hence enable AChE-R to affect both sperm differentiation and function by participating in independent cellular pathways.
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