ArticleGlycoconjugate journal2008
Serum antibody screening by surface plasmon resonance using a natural glycan microarray.
Article in Glycoconjugate journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 100 citations in OpenAlex.
- Novel Click Coupling Chemistry to Explore Glycan Recognition.ACS central science · 2025Article
- Serum antibody screening using glycan arrays.Chemical Society reviews · 2024Review
- Plasmon- and Waveguide-Coupled Fluorescence at the Ultraviolet Region.The journal of physical chemistry. C, Nanomaterials and interfaces · 2023Article
- Emergent Biosensing Technologies Based on Fluorescence Spectroscopy and Surface Plasmon Resonance.Sensors (Basel, Switzerland) · 2021Review
- Unique repertoire of anti-carbohydrate antibodies in individual human serum.Scientific reports · 2020Article
- On-Chip Neo-Glycopeptide Synthesis for Multivalent Glycan Presentation.Chemistry (Weinheim an der Bergstrasse, Germany) · 2020Article
- Resin and Magnetic Nanoparticle-Based Free Radical Probes for Glycan Capture, Isolation, and Structural Characterization.Analytical chemistry · 2019Article
- Factors contributing to variability of glycan microarray binding profiles.Faraday discussions · 2019Article
- Review
- "Stuck on sugars - how carbohydrates regulate cell adhesion, recognition, and signaling".Glycoconjugate journal · 2019Article
- Association between anti-α-1,4-D-polygalacturonic acid antibodies and Henoch-Schönlein purpura in children.The Journal of international medical research · 2019Observational
- Natural and Synthetic Sialylated Glycan Microarrays and Their Applications.Frontiers in molecular biosciences · 2019Review
- Repertoire of Abs primed by bacteria in gnotobiotic mice.Innate immunity · 2018Article
- Immobilization of glycans on solid surfaces for application in glycomics.Journal of carbohydrate chemistry · 2018Article
- Identification of Antigenic Glycans from Schistosoma mansoni by Using a Shotgun Egg Glycan Microarray.Infection and immunity · 2016Article
- Factors Affecting Anti-Glycan IgG and IgM Repertoires in Human Serum.Scientific reports · 2016Article
- Glycan microarrays of fluorescently-tagged natural glycans.Glycoconjugate journal · 2015Review
- Rhamnose glycoconjugates for the recruitment of endogenous anti-carbohydrate antibodies to tumor cells.Chembiochem : a European journal of chemical biology · 2014Article
- Differential expression of anti-glycan antibodies in schistosome-infected humans, rhesus monkeys and mice.Glycobiology · 2014Article
- Surface plasmon resonance applications in clinical analysis.Analytical and bioanalytical chemistry · 2014Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A surface plasmon resonance (SPR) based natural glycan microarray was developed for screening of interactions between glycans and carbohydrate-binding proteins (CBPs). The microarray contained 144 glycan samples and allowed the real-time and simultaneous screening for recognition by CBPs without the need of fluorescent labeling. Glycans were released from their natural source and coupled by reductive amination with the fluorescent labels 2-aminobenzamide (2AB) or anthranilic acid (AA) followed by high-performance liquid chromatography (HPLC) fractionation making use of the fluorescent tag. The released and labeled glycans, in addition to fluorescently labeled synthetic glycans and (neo)glycoproteins, were printed on an epoxide-activated chip at fmol amounts. This resulted in covalent immobilization, with the epoxide groups forming covalent bonds to the secondary amine groups present on the fluorescent glycoconjugates. The generated SPR glycan array presented a subset of the glycan repertoire of the human parasite Schistosoma mansoni. In order to demonstrate the usefulness of the array in the simultaneous detection of glycan-specific serum antibodies, the anti-glycan antibody profiles from sera of S. mansoni-infected individuals as well as from non-endemic uninfected controls were recorded. The SPR screening was sensitive for differences between infection sera and control sera, and revealed antibody titers and antibody classes (IgG or IgM). All SPR analyses were performed with a single SPR array chip, which required regeneration and blocking of the chip before the application of a serum sample. Our results indicate that SPR-based arrays constructed from glycans of natural or synthetic origin, pure or as mixture, can be used for determining serum antibody profiles as possible markers for the infection status of an individual.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.