ArticleApplied and environmental microbiology2008
Two different tetracycline resistance mechanisms, plasmid-carried tet(L) and chromosomally located transposon-associated tet(M), coexist in Lactobacillus sakei Rits 9.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed.
- Mineral-Based Advanced Oxidation Processes for Enhancing the Removal of Antibiotic Resistance Genes from Domestic Wastewater.ACS ES&T water · 2025Article
- Whole genome sequencing of promising Lactobacillus delbrueckii subsp. bulgaricus strains isolated from Egyptian dairy products for probiotic characteristics.Scientific reports · 2025Article
- Complete genome sequencing and probiotic characterization of promising lactic acid bacterial strains isolated from dairy products in Egyptian markets.BMC microbiology · 2025Article
- Comprehensive genomic assessment of available probiotics in India: A framework for safety and functionality evaluation.Bioinformation · 2025Article
- Serious Risk of Tigecycline Resistance in Escherichia coli Isolated from Swine Manure.Microbial ecology · 2023Article
- Antimicrobial susceptibility testing and tentative epidemiological cut-off values forFrontiers in antibiotics · 2023Article
- Genome analysis of probiotic bacteria for antibiotic resistance genes.Antonie van Leeuwenhoek · 2022Article
- Prevalence and Diversity of Antibiotic ResistantFrontiers in public health · 2022Article
- Assessing the drug resistance profiles of oral probiotic lozenges.Journal of oral microbiology · 2022Article
- Incidence of Tetracycline and Erythromycin Resistance in Meat-Associated Bacteria: Impact of Different Livestock Management Strategies.Microorganisms · 2021Article
- Exploring Antibiotic Resistance Diversity inMicroorganisms · 2021Article
- Safety Evaluation and Whole-Genome Annotation of Lactobacillus plantarum Strains from Different Sources with Special Focus on Isolates from Green Tea.Probiotics and antimicrobial proteins · 2020Article
- Genetic and Phenotypic Characteristics of a Multi-strain Probiotic for Broilers.Current microbiology · 2020Article
- Mobilome and Resistome Reconstruction from Genomes Belonging to Members of theMicroorganisms · 2019Article
- Antibiotic Resistance of LACTOBACILLUS Strains.Current microbiology · 2019Article
- Relationship between tetracycline antibiotic susceptibility and genotype in oral cavityAntimicrobial resistance and infection control · 2019Article
- Genus-Wide Assessment of Antibiotic Resistance inApplied and environmental microbiology · 2019Article
- Characterization of Erythromycin and Tetracycline Resistance inInternational journal of microbiology · 2018Article
- Lactobacillus sakei: A Starter for Sausage Fermentation, a Protective Culture for Meat Products.Microorganisms · 2017Review
- Assessment of antibiotic susceptibility inGut pathogens · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lactobacillus sakei is extensively used as functional starter culture in fermented meat products. One of the safety criteria of a starter culture is the absence of potentially transferable antibiotic resistance determinants. However, tetracycline-resistant L. sakei strains have already been observed. In this paper, we show that tetracycline resistance in L. sakei Rits 9, a strain isolated from Italian Sola cheese made from raw milk, is mediated by a transposon-associated tet(M) gene coding for a ribosomal protection protein and a plasmid-carried tet(L) gene coding for a tetracycline efflux pump. pLS55, the 5-kb plasmid carrying the tet(L) gene, is highly similar to the pMA67 plasmid recently described for Paenibacillus larvae, a species pathogenic to honeybees. pLS55 could be transferred by electroporation into the laboratory strain L. sakei 23K. While the L. sakei 23K transformant containing pLS55 displayed an intermediate tetracycline resistance level (MIC, <32 microg/ml), L. sakei Rits 9, containing both tetracycline-resistant determinants, had a MIC of <256 microg/ml, suggesting that Tet L and Tet M confer different levels of resistance in L. sakei. Remarkably, in the absence of tetracycline, a basal expression of both genes was detected for L. sakei Rits 9. In addition, subinhibitory concentrations of tetracycline affected the expression patterns of tet(M) and tet(L) in different ways: the expression of tet(M) was induced only at high tetracycline concentrations, whereas the expression of tet(L) was up-regulated at lower concentrations. This is the first time that two different mechanisms conferring resistance to tetracycline are characterized for the same strain of a lactic acid bacterium.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.