ArticleJournal of bacteriology2008
Regulation of type IV secretion apparatus genes during Ehrlichia chaffeensis intracellular development by a previously unidentified protein.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
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Who cites it
41 citing papers in PubMed, 58 citations in OpenAlex.
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- Glutathione Synthesis Regulated by CtrA ProtectsFrontiers in microbiology · 2022Article
- Insights into the mechanism regulating the differential expression of the P28-OMP outer membrane proteins in obligatory intracellular pathogenEmerging microbes & infections · 2021Article
- The super repertoire of type IV effectors in the pangenome of Ehrlichia spp. provides insights into host-specificity and pathogenesis.PLoS computational biology · 2021Article
- Iron robbery by intracellular pathogen via bacterial effector-induced ferritinophagy.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Sensing, Signaling, and Secretion: A Review and Analysis of Systems for Regulating Host Interaction inGenes · 2020Review
- PutA Is Required for Virulence and Regulated by PruR inFrontiers in microbiology · 2018Article
- Analysis of complete genome sequence and major surface antigens of Neorickettsia helminthoeca, causative agent of salmon poisoning disease.Microbial biotechnology · 2017Article
- Iron Starvation Conditions UpregulateFrontiers in cellular and infection microbiology · 2017Article
- Ehrlichia secretes Etf-1 to induce autophagy and capture nutrients for its growth through RAB5 and class III phosphatidylinositol 3-kinase.Autophagy · 2016Article
- Sequence determinants spanning -35 motif and AT-rich spacer region impacting Ehrlichia chaffeensis Sigma 70-dependent promoter activity of two differentially expressed p28 outer membrane protein genes.DNA research : an international journal for rapid publication of reports on genes and genomes · 2016Article
- Hacker within! Ehrlichia chaffeensis Effector Driven Phagocyte Reprogramming Strategy.Frontiers in cellular and infection microbiology · 2016Review
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- Secretome of obligate intracellular Rickettsia.FEMS microbiology reviews · 2015Review
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- Proteomic profiling of a robust Wolbachia infection in an Aedes albopictus mosquito cell line.Molecular microbiology · 2014Article
- Ehrlichia moonlighting effectors and interkingdom interactions with the mononuclear phagocyte.Microbes and infection · 2013Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The type IV secretion (T4S) system is critical for the virulence of several pathogens. In the rickettsial pathogen Ehrlichia chaffeensis, the virBD genes are split into two operons, the virB3-virB6 (preceded by sodB) and virB8-virD4 operons. Between these two operons, there are duplications of virB4, virB8, and virB9. In this study we found that transcription of all five loci was downregulated prior to the release of E. chaffeensis from host THP-1 cells and was upregulated at the initiation of exponential growth. Electrophoretic mobility shift assays revealed an E. chaffeensis-encoded protein that specifically bound to the promoter regions upstream of the virBD loci. The protein was purified from the bacterial lysate by affinity chromatography using a biotinylated promoter region upstream of sodB. Mass spectrometry identified the protein as an E. chaffeensis 12.3-kDa hypothetical protein, which was designated EcxR. Recombinant EcxR bound to the promoter regions upstream of five individual virBD loci. EcxR also activated transcription of all five virBD loci in lacZ reporter constructs. The expression of ecxR was positively autoregulated by EcxR. These results suggest that the five virBD loci are coordinately regulated by EcxR to allow developmental stage-specific expression of the T4S system in E. chaffeensis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.