ArticleBiophysical journal2008
Cytoplasmic force gradient in migrating adhesive cells.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- Compartmentalized cytoplasmic tradewinds direct soluble proteins.Nature communications · 2026Article
- The cytoplasm of living cells can sustain transient and steady intracellular pressure gradients.eLife · 2026Article
- Motor-Assisted Co-Migration of Intracellular Organelles and Microtubules as a Mechanism for Directed Cargo Transport.BioEssays : news and reviews in molecular, cellular and developmental biology · 2026Review
- G-actin diffusion is insufficient to achieve F-actin assembly in fast-treadmilling protrusions.Biophysical journal · 2023Article
- Intracellular production of hydrogels and synthetic RNA granules by multivalent molecular interactions.Nature materials · 2018Article
- Synaptopodin couples epithelial contractility to α-actinin-4-dependent junction maturation.The Journal of cell biology · 2015Article
- Bioengineering paradigms for cell migration in confined microenvironments.Current opinion in cell biology · 2014Review
- Article
- Article
- A POROELASTIC MODEL FOR CELL CRAWLING INCLUDING MECHANICAL COUPLING BETWEEN CYTOSKELETAL CONTRACTION AND ACTIN POLYMERIZATION.Journal of mechanics of materials and structures · 2011Article
- Forming the cell rear first: breaking cell symmetry to trigger directed cell migration.Nature cell biology · 2010Article
- Intracellular fluid flow in rapidly moving cells.Nature cell biology · 2009Article
- Article
- Cell biology of embryonic migration.Birth defects research. Part C, Embryo today : reviews · 2008Review
- Control of cell migration through mRNA localization and local translation.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Amoeboid movement is believed to involve a pressure gradient along the cell length, with contractions in the posterior region driving cytoplasmic streaming forward. However, a parallel mechanism has yet to be demonstrated in migrating adhesive cells. To probe the distribution of intracellular forces, we microinjected high molecular weight linear polyacrylamide (PAA) as a passive force sensor into migrating NIH3T3 fibroblasts. Injected PAA appeared as amorphous aggregates that underwent shape change and directional movement in response to differential forces exerted by the surrounding environment. PAA injected into the posterior region moved toward the front, whereas PAA in the anterior region never moved to the posterior region. This preferential forward movement was observed only in migrating cells with a defined polarity. Disruption of myosin II activity by blebbistatin inhibited the forward translocation of PAA while cell migration persisted in a disorganized fashion. These results suggest a myosin II-dependent force gradient in migrating cells, possibly as a result of differential cortical contractions between the anterior and posterior regions. This gradient may be responsible for the forward transport of cellular components and for maintaining the directionality during cell migration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.