ArticleThe Journal of biological chemistry2008
Cross-competition in editing of chloroplast RNA transcripts in vitro implicates sharing of trans-factors between different C targets.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Emergence of Novel RNA-Editing Sites by Changes in the Binding Affinity of a Conserved PPR Protein.Molecular biology and evolution · 2022Article
- Identification of soybean trans-factors associated with plastid RNA editing sites.Genetics and molecular biology · 2020Article
- Identification and analysis of RNA editing sites in chloroplast transcripts of kenaf (3 Biotech · 2019Article
- A pentatricopeptide repeat protein acts as a site-specificity factor at multiple RNA editing sites with unrelated cis-acting elements in plastids.Nucleic acids research · 2012Article
- Expression of plastid genes: organelle-specific elaborations on a prokaryotic scaffold.Plant physiology · 2011Article
- When you can't trust the DNA: RNA editing changes transcript sequences.Cellular and molecular life sciences : CMLS · 2011Review
- The RNA editing pattern of cox2 mRNA is affected by point mutations in plant mitochondria.PloS one · 2011Article
- MEF9, an E-subclass pentatricopeptide repeat protein, is required for an RNA editing event in the nad7 transcript in mitochondria of Arabidopsis.Plant physiology · 2010Article
- Distinct roles for sequences upstream of and downstream from Physarum editing sites.RNA (New York, N.Y.) · 2009Article
- Loss of matK RNA editing in seed plant chloroplasts.BMC evolutionary biology · 2009Article
- A comparative genomics approach identifies a PPR-DYW protein that is essential for C-to-U editing of the Arabidopsis chloroplast accD transcript.RNA (New York, N.Y.) · 2009Article
- CURE-Chloroplast: a chloroplast C-to-U RNA editing predictor for seed plants.BMC bioinformatics · 2009Article
- Post-transcriptional control of chloroplast gene expression.Gene regulation and systems biology · 2009Article
- A DYW domain-containing pentatricopeptide repeat protein is required for RNA editing at multiple sites in mitochondria of Arabidopsis thaliana.The Plant cell · 2009Article
- Multiple specificity recognition motifs enhance plant mitochondrial RNA editing in vitro.The Journal of biological chemistry · 2008Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
C-->U plant organellar RNA editing is required for the translation of evolutionarily conserved and functional proteins. 28 different C targets of RNA editing have been identified in maize chloroplasts, and hundreds of Cs are edited in mitochondria. Mutant analysis in Arabidopsis has indicated that absence of a single site-specific recognition protein can result in loss of editing of a single C target, raising the possibility that each C target requires a recognition protein. Here we show that transcripts encompassing two editing sites, ZMrpoB C467 and ZMrps14 C80, can compete editing activity from each other in vitro despite limited sequence similarity. The signal causing competition overlaps a 5'-cis element required for editing efficiency. A single five-nucleotide mutation spanning the region from -20 to -16 relative to the edited C of rpoB C467 is sufficient to eliminate its substrate editing as well as its ability to compete editing activity from rps14 C80 substrates. A corresponding mutation in an rps14 C80 competitor likewise eliminated its ability to compete editing activity from rpoB C467 substrates. Taken together, our results indicate that the RNA sequences mediating both editing efficiency and cross-competition are highly similar and that a common protein is involved in their editing. Sharing of trans-factors can facilitate editing of the large number of different C targets in plant organelles so that a different protein factor would not be required for every editing site.
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