ArticleCell host & microbe2008
Bacterial actin assembly requires toca-1 to relieve N-wasp autoinhibition.
Article in Cell host & microbe, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 61 citations in OpenAlex.
- Article
- ARP2/3 regulates cell surface dynamics of CeTOCA-1 inJournal of biosciences · 2025Article
- RACK1 promotesiScience · 2023Article
- Review
- The T3SS ofMicroorganisms · 2020Review
- BAR domain proteins-a linkage between cellular membranes, signaling pathways, and the actin cytoskeleton.Biophysical reviews · 2018Review
- Pathways of host cell exit by intracellular pathogens.Microbial cell (Graz, Austria) · 2018Review
- Identification of interactions among host and bacterial proteins and evaluation of their role early during Shigella flexneri infection.Microbiology (Reading, England) · 2018Article
- Actin-based motility and cell-to-cell spread of bacterial pathogens.Current opinion in microbiology · 2017Review
- Molecular and Cellular Mechanisms of Shigella flexneri Dissemination.Frontiers in cellular and infection microbiology · 2016Review
- Interactions between Shigella flexneri and the Autophagy Machinery.Frontiers in cellular and infection microbiology · 2016Review
- Shigella manipulates host immune responses by delivering effector proteins with specific roles.Frontiers in immunology · 2015Review
- Multiple roles of the cytoskeleton in bacterial autophagy.PLoS pathogens · 2014Review
- Article
- Host and bacterial proteins that repress recruitment of LC3 to Shigella early during infection.PloS one · 2014Article
- The inside story of Shigella invasion of intestinal epithelial cells.Cold Spring Harbor perspectives in medicine · 2013Review
- Arp2/3-mediated actin-based motility: a tail of pathogen abuse.Cell host & microbe · 2013Review
- Review
- Autophagy and bacterial clearance: a not so clear picture.Cellular microbiology · 2013Review
- Bruton's tyrosine kinase regulates Shigella flexneri dissemination in HT-29 intestinal cells.Infection and immunity · 2013Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Actin polymerization in the mammalian cytosol can be locally activated by mechanisms that relieve the autoinhibited state of N-WASP, an initiator of actin assembly, a process that also requires the protein Toca-1. Several pathogenic bacteria, including Shigella, exploit this host feature to infect and disseminate efficiently. The Shigella outer membrane protein IcsA recruits N-WASP, which upon activation at the bacterial surface mediates localized actin polymerization. The molecular role of Toca-1 in N-WASP activation during physiological or pathological actin assembly processes in intact mammalian cells remains unclear. We show that actin tail initiation by S. flexneri requires Toca-1 for the conversion of N-WASP from a closed inactive conformation to an open active one. While N-WASP recruitment is dependent on IcsA, Toca-1 recruitment is instead mediated by S. flexneri type III secretion effectors. Thus, S. flexneri independently hijacks two nodes of the N-WASP actin assembly pathway to initiate localized actin tail assembly.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.