Evidence map›Paper›PMID 18191414›Full record

ArticleJournal of immunological methods2008

In vivo validation of signaling pathways regulating human monocyte chemotaxis.

Ashish Bhattacharjee, Ravi S Mishra, Gerald M Feldman, Martha K Cathcart

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of immunological methods, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.3field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. iPLA2beta: front and center in human monocyte chemotaxis to MCP-1.The Journal of experimental medicine · 2008
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 1 country.

Ashish BhattacharjeeDepartment of Cell Biology, Cleveland Clinic, Cleveland, OH 44195, USA.
Ravi S Mishra
Gerald M Feldman
Martha K Cathcart
Cleveland Clinic · USCenter for Biologics Evaluation and Research · USCleveland Clinic Lerner College of Medicine · US

Funding

REGULATION OF MONOCYTE 15-LIPOXYGENASE EXPRESSIONP01HL087018 · NHLBI · CLEVELAND CLINIC LERNER COM-CWRU · PI MCINTYRE, THOMAS M · 2007 to 2011
$11.4M
VIRALLY-INDUCED STAT-1, STAT-3 AND STAT-6M01RR018390 · NCRR · CLEVELAND CLINIC LERNER COM-CWRU · PI CULVER, DANIEL A · 2003 to 2007
$11.0M
MONOCYTE PATHOGENESIS--RESPIRATORY BURST OXIDASER01HL061971 · NHLBI · CLEVELAND CLINIC LERNER COM-CWRU · PI CATHCART, MARTHA K · 1999 to 2007
$2.1M
PHOSPHOLIPASE REGULATION OF MONOCYTE CHEMOTAXIS TO MCP-1R01HL074451 · NHLBI · CLEVELAND CLINIC LERNER COM-CWRU · PI CATHCART, MARTHA K · 2003 to 2006
$1.4M
MONOCYTE OXIDATION OF LIPOPROTEIN LIPIDSR01HL051068 · NHLBI · CLEVELAND CLINIC FOUNDATION · PI CATHCART, MARTHA K · 1995 to 2002
$778k
NCRR NIH HHS M01 RR 018390NCRR NIH HHS M01 RR018390NHLBI NIH HHS HL 61971NHLBI NIH HHS HL 74451NHLBI NIH HHS P01 HL087018NHLBI NIH HHS R01 HL051068NHLBI NIH HHS R01 HL061971NHLBI NIH HHS R01 HL074451
6 · The paper itself

Abstract

Identification of novel signal transduction pathways regulating monocyte chemotaxis can indicate unique targets for preventive therapies for treatment of chronic inflammatory diseases. To aid in this endeavor we report conditions for optimal transfection of primary human monocytes coupled with a new model system for assessing their chemotactic activity in vivo. This method can be used as a tool to identify the relevant signal transduction pathways regulating human monocyte chemotaxis to MCP-1 in the complex in vivo environment that were previously identified to regulate chemotaxis in vitro. MCP-1-dependent chemotaxis of monocytes is studied in an adoptive transfer model where human monocytes transfected with mutant cDNAs are transferred to mice followed by initiation of peritonitis. Harvesting peritoneal cells at 24 h diminishes the contribution of immunologic responses to the cross-species transfer. Validation of relevant regulatory molecules in vivo is critical for understanding the most relevant therapeutic targets for drug development.

Indexed as

Chemotaxis, LeukocyteAdoptive TransferAnimalsBiological AssayCell MovementCells, CulturedCell SurvivalChemokine CCL2Disease Models, AnimalFeasibility StudiesGreen Fluorescent ProteinsHumansMiceMonocytesMutationPeritonitisCCL2 protein, humanChemokine CCL2Green Fluorescent ProteinsProtein Kinase CProtein Kinase C betaRecombinant Fusion ProteinsThioglycolates

Identifiers

PMID18191414
PMCPMC2292102
OpenAlexW1991104486

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.