ArticleDiagnostic microbiology and infectious disease2008
A line immunoassay utilizing recombinant nucleocapsid proteins for detection of antibodies to human coronaviruses.
Article in Diagnostic microbiology and infectious disease, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 31 citations in OpenAlex.
- A systematic review of antibody mediated immunity to coronaviruses: kinetics, correlates of protection, and association with severity.Nature communications · 2020Pooled it
- Seroprevalence of endemic and emergent coronaviruses among SARS-COV-2 patients and healthcare workers in Abidjan, Côte d'Ivoire.BMC infectious diseases · 2026Article
- SARS-CoV-2-specicific humoral immunity in convalescent patients with mild COVID-19 is supported by CD4Immunology letters · 2022Article
- Recent advances in immunoassay technologies for the detection of human coronavirus infections.Frontiers in cellular and infection microbiology · 2022Review
- Atypical Antibody Dynamics During Human Coronavirus HKU1 Infections.Frontiers in microbiology · 2022Article
- Seasonal Coronaviruses and Other Neglected Respiratory Viruses: A Global Perspective and a Local Snapshot.Frontiers in public health · 2021Review
- Seasonal coronavirus protective immunity is short-lasting.Nature medicine · 2020Article
- Epidemiology of Seasonal Coronaviruses: Establishing the Context for the Emergence of Coronavirus Disease 2019.The Journal of infectious diseases · 2020Article
- A systematic review of antibody mediated immunity to coronaviruses: antibody kinetics, correlates of protection, and association of antibody responses with severity of disease.medRxiv : the preprint server for health sciences · 2020Article
- Subunit Vaccines Against Emerging Pathogenic Human Coronaviruses.Frontiers in microbiology · 2020Review
- Serological Evidence of Bat SARS-Related Coronavirus Infection in Humans, China.Virologica Sinica · 2018Article
- A Melting Curve-Based Multiplex RT-qPCR Assay for Simultaneous Detection of Four Human Coronaviruses.International journal of molecular sciences · 2016Article
- First infection by all four non-severe acute respiratory syndrome human coronaviruses takes place during childhood.BMC infectious diseases · 2013Article
- Immunoreactivity characterisation of the three structural regions of the human coronavirus OC43 nucleocapsid protein by Western blot: implications for the diagnosis of coronavirus infection.Journal of virological methods · 2013Article
- The dominance of human coronavirus OC43 and NL63 infections in infants.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2012Article
- Development of a recombinant truncated nucleocapsid protein based immunoassay for detection of antibodies against human coronavirus OC43.Journal of virological methods · 2011Article
- Differentiation between human coronaviruses NL63 and 229E using a novel double-antibody sandwich enzyme-linked immunosorbent assay based on specific monoclonal antibodies.Clinical and vaccine immunology : CVI · 2011Article
- More and More Coronaviruses: Human Coronavirus HKU1.Viruses · 2009Article
- Development of a nucleocapsid-based human coronavirus immunoassay and estimates of individuals exposed to coronavirus in a U.S. metropolitan population.Clinical and vaccine immunology : CVI · 2008Article
- Human coronavirus NL63 and 229E seroconversion in children.Journal of clinical microbiology · 2008Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most coronaviruses infecting humans cause mild diseases, whereas severe acute respiratory syndrome (SARS)-associated coronavirus is an extremely dangerous pathogen. Here, we report the development of a serologic assay for detection of antibodies to human coronaviruses (HCoVs) based on recombinant nucleocapsid (N) proteins of all known pathogenic strains (229E, NL63, OC43, HKU1, SARS). The novel immunoassay is highly useful for epidemiologic surveys, where use of nucleic acid diagnostics often is limited. Purified recombinant antigens were immobilized on nitrocellulose membranes and applied in a line immunoassay, which allows rapid detection of antibodies to 5 different HCoVs in a single experiment. For assay evaluation, serum samples from persons infected with 229E or OC43 (acute/convalescent), recovered SARS patients and healthy donors were analyzed. Screening for nucleocapsid (N)-specific immunoglobulin G (IgG) in convalescent sera reached 100% sensitivity. With this new technique, we found that recently identified NL63 and HKU1 contribute significantly to the overall spectrum of coronavirus infections. Possibly, cross-reactive antibody responses were observed using 229E and OC43 serum pairs. However, the potential of this assay could clearly be demonstrated employing SARS-positive serum samples, where nonspecific binding to nucleocapsids of other HCoVs was not observed. This coronavirus strain-specific line immunoassay represents a powerful tool for serologic diagnostics.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.