ArticlePlant methods2008
Assaying protein palmitoylation in plants.
Article in Plant methods, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Reversible S-palmitoylation of C4 protein encoded by TYLCCxV orchestrates geminiviral pathogenesis.Stress biology · 2026Article
- Protocol for the visualization and identification of S-palmitoylated proteins in HCT-116 cells using metabolic labeling via click chemistry.STAR protocols · 2025Article
- D6PK plasma membrane polarity requires a repeated CXX(X)P motif and PDK1-dependent phosphorylation.Nature plants · 2024Article
- Salicylic acid attenuates brassinosteroid signaling via protein de-S-acylation.The EMBO journal · 2023Article
- Protein S-acylation controls the subcellular localization and biological activity of PHYTOCHROME KINASE SUBSTRATE.The Plant cell · 2023Article
- Palmitoylation of γb protein directs a dynamic switch between Barley stripe mosaic virus replication and movement.The EMBO journal · 2022Article
- An atlas of Arabidopsis protein S-acylation reveals its widespread role in plant cell organization and function.Nature plants · 2022Article
- Protocol to quantify palmitoylation of cysteines in budding yeast.STAR protocols · 2022Article
- Protein S-acyltransferases and acyl protein thioesterases, regulation executors of protein S-acylation in plants.Frontiers in plant science · 2022Review
- An ABHD17-like hydrolase screening system to identify de-S-acylation enzymes of protein substrates in plant cells.The Plant cell · 2021Article
- Auxin-induced signaling protein nanoclustering contributes to cell polarity formation.Nature communications · 2020Article
- Genetic variation in ZmTIP1 contributes to root hair elongation and drought tolerance in maize.Plant biotechnology journal · 2020Article
- A tetraspanin gene regulating auxin response and affecting orchid perianth size and various plant developmental processes.Plant direct · 2019Article
- A plant Bro1 domain protein BRAF regulates multivesicular body biogenesis and membrane protein homeostasis.Nature communications · 2018Article
- S-acylation mediates Mungbean yellow mosaic virus AC4 localization to the plasma membrane and in turns gene silencing suppression.PLoS pathogens · 2018Article
- Potato Mop-Top Virus Co-Opts the Stress Sensor HIPP26 for Long-Distance Movement.Plant physiology · 2018Article
- A Lipid-Anchored NAC Transcription Factor Is Translocated into the Nucleus and ActivatesThe Plant cell · 2017Article
- Maleimide scavenging enhances determination of protein S-palmitoylation state in acyl-exchange methods.BioTechniques · 2017Article
- Progress toward Understanding Protein S-acylation: Prospective in Plants.Frontiers in plant science · 2017Review
- Palmitoylation of the Cysteine Residue in the DHHC Motif of a Palmitoyl Transferase Mediates Ca2+ Homeostasis in Aspergillus.PLoS genetics · 2016Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
backgroundProtein S-acylation (also known as palmitoylation) is the reversible post-translational addition of acyl lipids to cysteine residues in proteins through a thioester bond. It allows strong association with membranes. Whilst prediction methods for S-acylation exist, prediction is imperfect. Existing protocols for demonstrating the S-acylation of plant proteins are either laborious and time consuming or expensive.
resultsWe describe a biotin switch method for assaying the S-acylation of plant proteins. We demonstrate the technique by showing that the heterotrimeric G protein subunit AGG2 is S-acylated as predicted by mutagenesis experiments. We also show that a proportion of the Arabidopsis alpha-tubulin subunit pool is S-acylated in planta. This may account for the observed membrane association of plant microtubules. As alpha-tubulins are ubiquitously expressed they can potentially be used as a positive control for the S-acylation assay regardless of the cell type under study.
conclusionWe provide a robust biotin switch protocol that allows the rapid assay of protein S-acylation state in plants, using standard laboratory techniques and without the need for expensive or specialised equipment. We propose alpha-tubulin as a useful positive control for the protocol.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.