ArticleEMBO reports2008
Cell-cycle-dependent binding kinetics for the early endosomal tethering factor EEA1.
Article in EMBO reports, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 31 citations in OpenAlex.
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- Time- and Spectrally-Resolved Photoluminescence Study of Alloyed CdInternational journal of molecular sciences · 2022Article
- De novo formation of early endosomes during Rab5-to-Rab7a transition.Journal of cell science · 2021Article
- Endosomal binding kinetics of Eps15 and Hrs specifically regulate the degradation of RTKs.Scientific reports · 2017Article
- Review
- Characterization of VPS34-IN1, a selective inhibitor of Vps34, reveals that the phosphatidylinositol 3-phosphate-binding SGK3 protein kinase is a downstream target of class III phosphoinositide 3-kinase.The Biochemical journal · 2014Article
- Transcriptional profiling and dynamical regulation analysis identify potential kernel target genes of SCYL1-BP1 in HEK293T cells.Molecules and cells · 2014Article
- Inhibition of endocytic vesicle fusion by Plk1-mediated phosphorylation of vimentin during mitosis.Cell cycle (Georgetown, Tex.) · 2014Article
- Emerging regulators of endosomal dynamics during mitosis.Cell cycle (Georgetown, Tex.) · 2014Article
- The ubiquitin/proteasome system mediates entry and endosomal trafficking of Kaposi's sarcoma-associated herpesvirus in endothelial cells.PLoS pathogens · 2012Article
- Proteins on the move: insights gained from fluorescent protein technologies.Nature reviews. Molecular cell biology · 2011Review
- Ultrastructural characterization of giant endosomes induced by GTPase-deficient Rab5.Histochemistry and cell biology · 2010Article
- Photoactivatable fluorescent proteins for diffraction-limited and super-resolution imaging.Trends in cell biology · 2009Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Early endosomal antigen 1 (EEA1) is a cytosolic protein that specifically binds to early endosomal membranes where it has a crucial role in the tethering process leading to homotypic endosome fusion. Green fluorescent protein-tagged EEA1 (EEA1-GFP) was bound to the endosomal membrane throughout the cell cycle, and measurements using fluorescent recovery after photobleaching showed two fractions: one rapidly exchanging with the cytosolic pool, and the other with a long half-life. The exchange consists of a release and binding process, and we have separated these two by using GFP and photoactivable GFP. The release rate was identical to the exchange rate, showing that the dissociation characteristics determine the cycling of this molecule. During mitosis, we found that the dissociation rate was markedly accelerated and, in addition, the long-lived fraction was markedly reduced. This indicates that a fusion arrest in mitosis is not the result of EEA1 not binding to early endosomes, but rather due to the marked shift in membrane-binding characteristics. This might be a general mechanism to fine-tune and control tethering and fusion of early endosomes.
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