ArticleEndocrinology2008
Parathyroid hormone increases activating transcription factor 4 expression and activity in osteoblasts: requirement for osteocalcin gene expression.
Article in Endocrinology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 43 citations in OpenAlex.
- Critical role of AKT protein in myeloma-induced osteoclast formation and osteolysis.The Journal of biological chemistry · 2013Trial
- Vitamin K as an Endocrine Modulator: Mechanistic Links to Glucose Metabolism and Beyond.Nutrients · 2026Review
- Emerging roles for integrated stress response signaling in homeostasis.The FEBS journal · 2025Review
- Article
- MgCl2 promotes mouse mesenchymal stem cell osteogenic differentiation by activating the p38/Osx/Runx2 signaling pathway.Molecular medicine reports · 2020Article
- The loss of activating transcription factor 4 (ATF4) reduces bone toughness and fracture toughness.Bone · 2014Article
- ATF4 promotes bone angiogenesis by increasing VEGF expression and release in the bone environment.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2013Article
- ATF4 protein deficiency protects against high fructose-induced hypertriglyceridemia in mice.The Journal of biological chemistry · 2013Article
- Article
- PTHrP expression in human MDA-MB-231 breast cancer cells is critical for tumor growth and survival and osteoblast inhibition.International journal of biological sciences · 2013Article
- atf4 promotes β-catenin expression and osteoblastic differentiation of bone marrow mesenchymal stem cells.International journal of biological sciences · 2013Article
- Role of fibroblast growth factor 2 and Wnt signaling in anabolic effects of parathyroid hormone on bone formation.Journal of cellular physiology · 2012Review
- Si and Ca individually and combinatorially target enhanced MC3T3-E1 subclone 4 early osteogenic marker expression.The Journal of oral implantology · 2012Article
- Critical role of filamin-binding LIM protein 1 (FBLP-1)/migfilin in regulation of bone remodeling.The Journal of biological chemistry · 2012Article
- The basic helix loop helix transcription factor Twist1 is a novel regulator of ATF4 in osteoblasts.Journal of cellular biochemistry · 2012Article
- The impaired bone anabolic effect of PTH in the absence of endogenous FGF2 is partially due to reduced ATF4 expression.Biochemical and biophysical research communications · 2011Article
- Foxo1 mediates insulin-like growth factor 1 (IGF1)/insulin regulation of osteocalcin expression by antagonizing Runx2 in osteoblasts.The Journal of biological chemistry · 2011Article
- Activating transcription factor 4 regulates osteoclast differentiation in mice.The Journal of clinical investigation · 2010Article
- Fibroblast growth factor 2 positively regulates expression of activating transcription factor 4 in osteoblasts.Biochemical and biophysical research communications · 2010Article
- Atf4 regulates chondrocyte proliferation and differentiation during endochondral ossification by activating Ihh transcription.Development (Cambridge, England) · 2009Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
PTH is an important peptide hormone regulator of calcium homeostasis and osteoblast function. However, its mechanism of action in osteoblasts is poorly understood. Our previous study demonstrated that PTH activates mouse osteocalcin (Ocn) gene 2 promoter through the osteoblast-specific element 1 site, a recently identified activating transcription factor-4 (ATF4) -binding element. In the present study, we examined effects of PTH on ATF4 expression and activity as well as the requirement for ATF4 in the regulation of Ocn by PTH. Results show that PTH elevated levels of ATF4 mRNA and protein in a dose- and time-dependent manner. This PTH regulation requires transcriptional activity but not de novo protein synthesis. PTH also increased binding of nuclear extracts to osteoblast-specific element 1 DNA. PTH stimulated ATF4-dependent transcriptional activity mainly through protein kinase A with a lesser requirement for protein kinase C and MAPK/ERK pathways. Lastly, PTH stimulation of Ocn expression was lost by small interfering RNA down-regulation of ATF4 in MC-4 cells and Atf4(-/-) bone marrow stromal cells. Collectively, these studies for the first time demonstrate that PTH increases ATF4 expression and activity and that ATF4 is required for PTH induction of Ocn expression in osteoblasts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.