ArticleJournal of cell science2008
Paxillin-dependent stimulation of microtubule catastrophes at focal adhesion sites.
Article in Journal of cell science, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 60 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
60 citing papers in PubMed, 94 citations in OpenAlex.
- Septins guide noncentrosomal microtubules to promote focal adhesion disassembly in migrating cells.Molecular biology of the cell · 2022Article
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- LIM domain proteins in cell mechanobiology.Cytoskeleton (Hoboken, N.J.) · 2021Review
- Spatial regulation of MCAK promotes cell polarization and focal adhesion turnover to drive robust cell migration.Molecular biology of the cell · 2021Article
- T-Plastin reinforces membrane protrusions to bridge matrix gaps during cell migration.Nature communications · 2020Article
- Profilin 1 delivery tunes cytoskeletal dynamics toward CNS axon regeneration.The Journal of clinical investigation · 2020Article
- Paxillin family of focal adhesion adaptor proteins and regulation of cancer cell invasion.International review of cell and molecular biology · 2020Article
- The bioenergetics of integrin-based adhesion, from single molecule dynamics to stability of macromolecular complexes.Computational and structural biotechnology journal · 2020Review
- The focal adhesion scaffold protein Hic-5 regulates vimentin organization in fibroblasts.Molecular biology of the cell · 2019Article
- RITA Is Expressed in Trophoblastic Cells and Is Involved in Differentiation Processes of the Placenta.Cells · 2019Article
- Microtubules in cell migration.Essays in biochemistry · 2019Review
- The role of APC-mediated actin assembly in microtubule capture and focal adhesion turnover.The Journal of cell biology · 2019Article
- RITA modulates cell migration and invasion by affecting focal adhesion dynamics.Molecular oncology · 2019Article
- Gold Nanorod Photothermal Therapy Alters Cell Junctions and Actin Network in Inhibiting Cancer Cell Collective Migration.ACS nano · 2018Article
- Axonal neurofilaments exhibit frequent and complex folding behaviors.Cytoskeleton (Hoboken, N.J.) · 2018Article
- Kymograph analysis with high temporal resolution reveals new features of neurofilament transport kinetics.Cytoskeleton (Hoboken, N.J.) · 2018Article
- Sphingolipids Signaling in Lamellipodia Formation and Enhancement of Endothelial Barrier Function.Current topics in membranes · 2018Article
- Paxillin regulates cell polarization and anterograde vesicle trafficking during cell migration.Molecular biology of the cell · 2017Article
Corrections and comments
- Erratum issuedOhi, Ryoma [added]
Authors and funding
8 authors at 5 institutions in 3 countries.
Funding
Abstract
An organized microtubule array is essential for the polarized motility of fibroblasts. Dynamic microtubules closely interact with focal adhesion sites in migrating cells. Here, we examined the effect of focal adhesions on microtubule dynamics. We observed that the probability of microtubule catastrophes (transitions from growth to shrinkage) was seven times higher at focal adhesions than elsewhere. Analysis of the dependence between the microtubule growth rate and catastrophe probability throughout the cytoplasm revealed that a nonspecific (mechanical or spatial) factor provided a minor contribution to the catastrophe induction by decreasing microtubule growth rate at adhesions. Strikingly, at the same growth rate, the probability of catastrophes was significantly higher at adhesions than elsewhere, indicative of a site-specific biochemical trigger. The observed catastrophe induction occurred at adhesion domains containing the scaffolding protein paxillin that has been shown previously to interact with tubulin. Furthermore, replacement of full-length paxillin at adhesion sites by microinjected paxillin LIM2-LIM3 domains suppressed microtubule catastrophes exclusively at adhesions. We suggest that paxillin influences microtubule dynamics at focal adhesions by serving as a scaffold for a putative catastrophe factor and/or regulating its exposure to microtubules.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.