ArticleJournal of virological methods2008
Establishment of fetal bovine intestinal epithelial cell cultures susceptible to bovine rotavirus infection.
Article in Journal of virological methods, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- Establishment of bovine 3D enteroid-derived 2D monolayers.Veterinary research · 2022Article
- Isolation and development of bovine primary respiratory cells as model to study influenza D virus infection.Virology · 2021Article
- Characterization of a bovine intestinal myofibroblast cell line and stimulation using phytoglycogen-based nanoparticles bound to inosine monophosphate.In vitro cellular & developmental biology. Animal · 2021Article
- Assessing Intestinal Health.Frontiers in veterinary science · 2021Review
- Development and characterization of a stable bovine intestinal sub-epithelial myofibroblast cell line from ileum of a young calf.In vitro cellular & developmental biology. Animal · 2019Article
- Development and biochemical and immunological characterization of early passage and immortalized bovine intestinal epithelial cell lines from the ileum of a young calf.Cytotechnology · 2019Article
- Article
- Article
- Establishment and long-term maintenance of primary intestinal epithelial cells cultured from the rainbow trout,Biology open · 2018Article
- Development and characterization of 2-dimensional culture for buffalo intestinal cells.Cytotechnology · 2018Article
- Immunobiotics for the Bovine Host: Their Interaction with Intestinal Epithelial Cells and Their Effect on Antiviral Immunity.Frontiers in immunology · 2018Review
- Assessment of yeast Saccharomyces cerevisiae component binding to Mycobacterium avium subspecies paratuberculosis using bovine epithelial cells.BMC veterinary research · 2016Article
- Human alveolar epithelial type II cells in primary culture.Physiological reports · 2015Article
- Establishment of an in vitro culture system for intestinal epithelial cells from Pheretima aspergillum (E. Perrier).In vitro cellular & developmental biology. Animal · 2014Article
- Characteristic and functional analysis of a newly established porcine small intestinal epithelial cell line.PloS one · 2014Article
- Article
- Characterization of a porcine intestinal epithelial cell line for influenza virus production.The Journal of general virology · 2012Article
- Probiotic-Induced Priming of Innate Immunity to Protect Against Rotaviral Infection.Probiotics and antimicrobial proteins · 2010Article
- Characterization of newly established bovine intestinal epithelial cell line.Histochemistry and cell biology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mucosal epithelial cells are infected by a wide variety of pathogens and determining their response to infection is critical for understanding disease pathogenesis. A protocol was developed for culturing primary epithelial cells from fetal bovine intestine and the cultured cells were evaluated for susceptibility to an enteric viral infection. Immunohistochemical staining for cytokeratin confirmed that 60-75% of cultured cells were epithelial cells. Furthermore, following infection with bovine rotavirus (BRV) over 80% of cells in the ileal and jejunal cultures contained viral protein at 16 h post-infection. The intestinal epithelial cell cultures also contained fibroblasts so a jejunal fibroblast culture was established and infected with BRV. Viral protein was detected in jejunal fibroblasts but viral-induced cytopathology was delayed in fibroblast cultures when compared to epithelial cell cultures. This study describes an effective protocol for culturing bovine epithelial cells from fetal intestine and confirmed that the epithelial cells were susceptible to BRV infection. Ileal and jejunal cultures displayed limited growth following continuous passage but early passage epithelial cells provide competent target cells for studying host cell responses to an enteric viral pathogen.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.