ArticleThe British journal of nutrition2008
Feeding pregnant rats a protein-restricted diet persistently alters the methylation of specific cytosines in the hepatic PPAR alpha promoter of the offspring.
Article in The British journal of nutrition, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 167 papers.
What it found
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Who cites it
167 citing papers in PubMed, 447 citations in OpenAlex.
- ANRIL Promoter DNA Methylation: A Perinatal Marker for Later Adiposity.EBioMedicine · 2017Trial
- Hypermethylation at loci sensitive to the prenatal environment is associated with increased incidence of myocardial infarction.International journal of epidemiology · 2012Trial
- Neural stem cells as potential mediators of prenatal dietary stress through epigenetic mechanisms.Stem cell reports · 2026Article
- Epigenetic remodeling of sheep oocytes and embryos induced by maternal methionine supplementation.Epigenetics · 2025Article
- Promoter H3K4me3 and Gene Expression Involved in Systemic Metabolism Are Altered in Fetal Calf Liver of Nutrient-Restricted Dams.International journal of molecular sciences · 2025Article
- PlacentaAmerican journal of physiology. Endocrinology and metabolism · 2025Article
- MASLD: insights on the role of folate in hepatic lipid metabolism.Frontiers in nutrition · 2025Review
- Factors influencing brain recovery from stroke via possible epigenetic changes.Future science OA · 2024Review
- DNA Methylation of Genes Participating in Hepatic Metabolisms and Function in Fetal Calf Liver Is Altered by Maternal Undernutrition during Gestation.International journal of molecular sciences · 2023Article
- Growth-Restricted Fetuses and Offspring Reveal Adverse Sex-Specific Metabolic Responses in Preeclamptic Mice Expressing Human sFLT1.International journal of molecular sciences · 2023Article
- A low-protein maternal diet during gestation affects the expression of key pancreatic β-cell genes and the methylation status of the regulatory region of theFrontiers in veterinary science · 2023Article
- Dynamics and consequences of nutrition-related microbial dysbiosis in early life: study protocol of the VITERBI GUT project.Frontiers in nutrition · 2023Article
- Human non-CpG methylation patterns display both tissue-specific and inter-individual differences suggestive of underlying function.Epigenetics · 2022Article
- Human Milk Lipids Induce Important Metabolic and Epigenetic Changes in Neonates.Clinics in perinatology · 2022Review
- Maternal low protein diet and fetal programming of lean type 2 diabetes.World journal of diabetes · 2022Review
- The metabolic conditioning of obesity: A review of the pathogenesis of obesity and the epigenetic pathways that "program" obesity from conception.Frontiers in endocrinology · 2022Review
- Nutritional Epigenetics: How Metabolism Epigenetically Controls Cellular Physiology, Gene Expression and Disease.Sub-cellular biochemistry · 2022Article
- Nutritional Status Impacts Epigenetic Regulation in Early Embryo Development: A Scoping Review.Advances in nutrition (Bethesda, Md.) · 2021Article
- Modulation of PPAR signaling disrupts pancreas development in the zebrafish, Danio rerio.Toxicology and applied pharmacology · 2021Article
- Calcium-Deficiency during Pregnancy Affects Insulin Resistance in Offspring.International journal of molecular sciences · 2021Review
107 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Induction of an altered phenotype by prenatal under-nutrition involves changes in the epigenetic regulation of specific genes. We investigated the effect of feeding pregnant rats a protein-restricted (PR) diet with different amounts of folic acid on the methylation of individual CpG dinucleotides in the hepatic PPAR alpha promoter in juvenile offspring, and the effect of the maternal PR diet on CpG methylation in adult offspring. Pregnant rats (five per group) were fed 180 g/kg casein (control) or 90 g/kg casein with 1 mg/kg folic acid (PR), or 90 g/kg casein and 5 mg/kg folic acid (PRF). Offspring were killed on postnatal day 34 (five males and females per group) and day 80 (five males per group). Methylation of sixteen CpG dinucleotides in the PPAR alpha promoter was measured by pyrosequencing. Mean PPAR alpha promoter methylation in the PR offspring (4.5 %) was 26 % lower than controls (6.1 %) due to specific reduction at CpG dinucleotides 2 (40 %), 3 (43 %), 4 (33 %) and 16 (48 %) (P < 0.05). There was no significant difference in methylation at these CpG between control and PRF offspring. Methylation of CpG 5 and 8 was higher (47 and 63 %, respectively, P < 0.05) in the PRF offspring than control or PR offspring. The methylation pattern in day 80 PR offspring was comparable to day 34 PR offspring. These data show for the first time that prenatal nutrition induces differential changes to the methylation of individual CpG dinucleotides in juvenile rats which persist in adults.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.