ArticleArthritis research & therapy2008
CD47 associates with alpha 5 integrin and regulates responses of human articular chondrocytes to mechanical stimulation in an in vitro model.
Article in Arthritis research & therapy, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 42 citations in OpenAlex.
- CD47 signaling in aging and age-related diseases: mechanisms, challenges, and therapeutic opportunities.Biogerontology · 2025Review
- Integrin signaling pathways in mesenchymal stem cells.Stem cell research & therapy · 2025Review
- The mechanobiology of extracellular matrix: a focus on thrombospondins.Cell communication and signaling : CCS · 2025Review
- CD47 and thrombospondin-1 contribute to immune evasion byProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Deciphering the role of CD47 in cancer immunotherapy.Journal of advanced research · 2024Review
- Progress in cancer research on the regulator of phagocytosis CD47, which determines the fate of tumor cells (Review).Oncology letters · 2024Review
- A MD Simulation Prediction for Regulation ofMolecules (Basel, Switzerland) · 2023Article
- Nanoscale imaging of CD47 informs how plasma membrane modifications shape apoptotic cell recognition.Communications biology · 2023Article
- The landscape overview of CD47-based immunotherapy for hematological malignancies.Biomarker research · 2023Review
- Mechanosignalling in cartilage: an emerging target for the treatment of osteoarthritis.Nature reviews. Rheumatology · 2022Review
- Mechanistic Insight Into the Roles of Integrins in Osteoarthritis.Frontiers in cell and developmental biology · 2021Review
- Role of CD47 in Hematological Malignancies.Journal of hematology & oncology · 2020Review
- Mechanotransduction pathways in the regulation of cartilage chondrocyte homoeostasis.Journal of cellular and molecular medicine · 2020Review
- CD47: role in the immune system and application to cancer therapy.Cellular oncology (Dordrecht, Netherlands) · 2020Review
- Dysregulated integrin αVβ3 and CD47 signaling promotes joint inflammation, cartilage breakdown, and progression of osteoarthritis.JCI insight · 2019Article
- Extracellular matrix type modulates mechanotransduction of stem cells.Acta biomaterialia · 2019Article
- Role of integrins and their ligands in osteoarthritic cartilage.Rheumatology international · 2015Review
- Integrins and chondrocyte-matrix interactions in articular cartilage.Matrix biology : journal of the International Society for Matrix Biology · 2014Review
- Ubiquilin-2 (UBQLN2) binds with high affinity to the C-terminal region of TDP-43 and modulates TDP-43 levels in H4 cells: characterization of inhibition by nucleic acids and 4-aminoquinolines.Biochimica et biophysica acta · 2013Article
- CD47: A Cell Surface Glycoprotein Which Regulates Multiple Functions of Hematopoietic Cells in Health and Disease.ISRN hematology · 2013Article
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Authors and funding
7 authors at 4 institutions in 4 countries.
Funding
Abstract
backgroundRecent studies provide evidence of roles for integrins in mechanical signalling in bone and cartilage. Integrin signalling is modulated by various mechanisms, including interaction with other transmembrane proteins. We aimed to identify whether one such protein, integrin-associated protein (CD47/IAP), is expressed by chondrocytes and whether it may regulate integrin-dependent mechanotransduction.
methodsChondrocytes, isolated from macroscopically normal and osteoarthritic articular cartilage of human knee joints, were studied in a resting state or following mechanical stimulation at 0.33 Hz. CD47/IAP expression and associations were confirmed by immunohistology, reverse transcription-polymerase chain reaction, Western blotting, and immunoprecipitation. Roles in mechanotransduction were studied by assessing effects of function-blocking antibodies on a range of electrophysiological, cellular, and molecular responses of primary chondrocytes and responses of CD47/IAP-null cell lines transfected with CD47/IAP.
resultsHuman articular chondrocytes were shown to express CD47/IAP, predominantly the type 2 isoform. Immunoprecipitation showed association of CD47/IAP with alpha5 integrin and thrombospondin but not SIRPalpha (signal-regulatory protein-alpha). The function-blocking anti-CD47/IAP antibody Bric 126 inhibited changes in membrane potential, tyrosine phosphorylation, and elevation of relative levels of aggrecan mRNA induced by mechanical stimulation, whereas in the presence of B6H12, an antibody that has partial agonist activity, a membrane depolarisation rather than a membrane hyperpolarisation response was induced by mechanical stimulation. CD47-null cell lines did not show changes in cell membrane potential following mechanical stimulation. Changes in cell membrane potential following mechanical stimulation were seen when CD47-null cells were transfected with CD47/IAP expression vectors but were not seen following mechanical stimulation of cells transfected with vectors for the extracellular immunoglobulin variable (IgV) domain of CD47/IAP in the absence of the transmembrane and intracellular domains.
conclusionCD47/IAP is necessary for chondrocyte mechanotransduction. Through interactions with alpha5beta1 integrin and thrombospondin, CD47/IAP may modulate chondrocyte responses to mechanical signals.
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