ArticleWorld journal of gastroenterology2008
Expression of pituitary homeobox 1 gene in human gastric carcinogenesis and its clinicopathological significance.
Article in World journal of gastroenterology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 33 citations in OpenAlex.
- Genome-wide association analyses in East Asians identify new susceptibility loci for colorectal cancer.Nature genetics · 2013Pooled it
- PITX1 as a grading, prognostic and tumor-infiltrating immune cells marker for chondrosarcoma: a public database-based immunoassay and tissue sample analysis.Frontiers in oncology · 2025Article
- PITX1 plays essential functions in cancer.Frontiers in oncology · 2023Review
- PITX1 inhibits the growth and proliferation of melanoma cells through regulation of SOX family genes.Scientific reports · 2021Article
- Novel methylated DNA markers accurately discriminate Lynch syndrome associated colorectal neoplasia.Epigenomics · 2020Article
- Silenced PITX1 promotes chemotherapeutic resistance to 5-fluorocytosine and cisplatin in gastric cancer cells.Experimental and therapeutic medicine · 2019Article
- Article
- Interaction between physical activity,Oncotarget · 2018Article
- DNA hypermethyation and silencing ofOncotarget · 2017Article
- Clinicopathological features and pituitary homeobox 1 gene expression in the progression and prognosis of cutaneous malignant melanoma.The Kaohsiung journal of medical sciences · 2016Article
- PITX1 is a novel predictor of the response to chemotherapy in head and neck squamous cell carcinoma.Molecular and clinical oncology · 2016Article
- Review
- Strong expression of paired-like homeodomain transcription factor 1 (PITX1) is associated with a favorable outcome in human osteosarcoma.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2015Article
- miR-19b regulates hTERT mRNA expression through targeting PITX1 mRNA in melanoma cells.Scientific reports · 2015Article
- Identification of a Potential Regulatory Variant for Colorectal Cancer Risk Mapping to Chromosome 5q31.1: A Post-GWAS Study.PloS one · 2015Article
- The RNA helicase RHAU (DHX36) suppresses expression of the transcription factor PITX1.Nucleic acids research · 2014Article
- PITX1 is a reliable biomarker for predicting prognosis in patients with oral epithelial dysplasia.Oncology letters · 2014Article
- Single nucleotide polymorphisms associated with colorectal cancer susceptibility and loss of heterozygosity in a Taiwanese population.PloS one · 2014Article
- Loss of desmocollin 1-3 and homeobox genes PITX1 and CDX2 are associated with tumor progression and survival in colorectal carcinoma.International journal of colorectal disease · 2012Article
- Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo investigate the effect of pituitary homeobox 1 (PITX1) expression in cases of human gastric cancer on cancer differentiation and progression, and carcinogenesis.
methodsUsing polyclonal PITX1 antibodies, we studied the expression of PITX1 in normal gastric mucosa, atypical hyperplasia, intestinal metaplasia, and cancer tissue samples from 83 gastric cancer patients by immunohistochemistry. Moreover, semi-reverse transcription polymerase chain reaction (semi-RT-PCR) was performed to detect the mRNA level of PITX1 in three gastric cancer cell lines and a normal gastric epithelial cell line. Subsequently, somatic mutations of the PITX1 gene in 71 gastric cancer patients were analyzed by a combination of denaturing high performance liquid chromatography (DHPLC) and DNA sequencing.
resultsImmunohistochemistry showed that PITX1 was strongly or moderately expressed in the parietal cells of normal gastric mucosa (100%), while 55 (66.3%) out of 83 samples of gastric cancers showed decreased PITX1 expression. Moreover, PITX1 expression was reduced in 20 out of 28 cases (71.5%) of intestinal metaplasia, but in only 1 out of 9 cases (11%) of atypical hyperplasia. More importantly, PITX1 expression was significantly associated with the differentiation, position and invasion depth of gastric cancers (r = -0.316, P < 0.01; r = 0.213, P < 0.05; r = -0.259, P < 0.05, respectively). Similarly, levels of PITX1 mRNA were significantly decreased in 2 gastric cancer cell lines, BGC-823 and SGC-7901, compared with the normal gastric epithelial cell line GES-1 (0.306 +/- 0.060 vs 0.722 +/- 0.102, P < 0.05; 0.356 +/- 0.081 vs 0.722 +/- 0.102, P < 0.05, respectively). Nevertheless, no somatic mutation of PITX1 gene was found in 71 samples of gastric cancer by DHPLC analysis followed by sequencing.
conclusionDown-regulation of PITX1 may be a frequent molecular event in gastric carcinogenesis. Aberrant levels of PITX1 expression may be closely correlated with the progression and differentiation of gastric cancer.
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