ArticlePlant physiology2008
Farnesylation directs AtIPT3 subcellular localization and modulates cytokinin biosynthesis in Arabidopsis.
Article in Plant physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 72 citations in OpenAlex.
- Farnesol and geranylgeraniol in plant reproduction: insights from Arabidopsis and beyond.Journal of experimental botany · 2026Review
- Chloroplastic protein prenylation: an internal or external affair? Reconciling biochemical data through a new hypothesis.Frontiers in plant science · 2026Article
- Exploring the Cytokinin Profile ofMetabolites · 2025Article
- Protein Prenylation in Plants: Mechanisms and Functional Implications.Plants (Basel, Switzerland) · 2025Review
- Spatially distributed cytokinins: Metabolism, signaling, and transport.Plant communications · 2024Review
- Genome-wide characterization of heavy metal-associated isoprenylated plant protein gene family fromFrontiers in plant science · 2024Article
- The ectomycorrhizal fungusFrontiers in microbiology · 2024Article
- The LONELY GUY gene family: from mosses to wheat, the key to the formation of active cytokinins in plants.Plant biotechnology journal · 2022Review
- Genotypic-specific hormonal reprogramming and crosstalk are crucial for root growth and salt tolerance in bermudagrass (Frontiers in plant science · 2022Article
- The Hulks and the Deadpools of the Cytokinin Universe: A Dual Strategy for Cytokinin Production, Translocation, and Signal Transduction.Biomolecules · 2021Review
- Characterization of the Heavy-Metal-Associated Isoprenylated Plant Protein (International journal of molecular sciences · 2020Article
- Article
- New Insights Into the Metabolism and Role of CytokininFrontiers in plant science · 2020Review
- Occurrence and biosynthesis of cytokinins in poplar.Planta · 2019Article
- Sugar-induced de novo cytokinin biosynthesis contributes to Arabidopsis growth under elevated COScientific reports · 2019Article
- Cytokinin Transporters: Multisite Players in Cytokinin Homeostasis and Signal Distribution.Frontiers in plant science · 2019Review
- Auxins and Cytokinins-The Role of Subcellular Organization on Homeostasis.International journal of molecular sciences · 2018Review
- Cytokinin Biosynthesis Promotes Cortical Cell Responses during Nodule Development.Plant physiology · 2017Article
- Subcellular localization of Arabidopsis arogenate dehydratases suggests novel and non-enzymatic roles.Journal of experimental botany · 2017Article
- Tomato (Solanum lycopersicum L.) SlIPT3 and SlIPT4 isopentenyltransferases mediate salt stress response in tomato.BMC plant biology · 2015Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytokinins regulate cell division and differentiation as well as a number of other processes implicated in plant development. The first step of cytokinin biosynthesis in Arabidopsis (Arabidopsis thaliana) is catalyzed by adenosine phosphate-isopentenyltransferases (AtIPT). The enzymes are localized in plastids or the cytoplasm where they utilize the intermediate dimethylallyl-diphosphate from the methylerythritolphosphate or mevalonic acid pathways. However, the regulatory mechanisms linking AtIPT activity and cytokinin biosynthesis with cytokinin homeostasis and isoprenoid synthesis are not well understood. Here, we demonstrate that expression of AtIPT3, one member of the adenosine AtIPT protein family in Arabidopsis, increased the production of specific isopentenyl-type cytokinins. Moreover, AtIPT3 is a substrate of the protein farnesyl transferase, and AtIPT3 farnesylation directed the localization of the protein in the nucleus/cytoplasm, whereas the nonfarnesylated protein was located in the plastids. AtIPT3 gain-of-function mutant analysis indicated that the different subcellular localization of the farnesylated protein and the nonfarnesylated protein was closely correlated with either isopentenyl-type or zeatin-type cytokinin biosynthesis. In addition, mutation of the farnesyl acceptor cysteine-333 of AtIPT3 abolishes cytokinin production, suggesting that cysteine-333 has a dual and essential role for AtIPT3 farnesylation and catalytic activity.
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