ArticlePlant physiology2008
The plant defense elicitor cryptogein stimulates clathrin-mediated endocytosis correlated with reactive oxygen species production in bright yellow-2 tobacco cells.
Article in Plant physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 85 citations in OpenAlex.
- SOBIR1 integrates pathogen recognition and hormonal metabolism in Nicotiana tabacum.Plant physiology · 2026Article
- Refractory Behavior in Plant Cells-Calcium Signaling Induced by Biotic Stress.Plants (Basel, Switzerland) · 2026Review
- Plant elicitor peptide induces endocytosis of plasma membrane proteins in Arabidopsis.Frontiers in plant science · 2023Article
- Unraveling Plant Cell Death duringMicroorganisms · 2022Review
- Apoplastic Cell Death-Inducing Proteins of Filamentous Plant Pathogens: Roles in Plant-Pathogen Interactions.Frontiers in genetics · 2020Review
- Drought stress stimulates endocytosis and modifies membrane lipid order of rhizodermal cells of Medicago truncatula in a genotype-dependent manner.BMC plant biology · 2019Article
- Osmotic Stress Activates Two Reactive Oxygen Species Pathways with Distinct Effects on Protein Nanodomains and Diffusion.Plant physiology · 2019Article
- Lipid transfer proteins: the lipid commute via shuttles, bridges and tubes.Nature reviews. Molecular cell biology · 2019Review
- Chloroplasts at the Crossroad of Photosynthesis, Pathogen Infection and Plant Defense.International journal of molecular sciences · 2018Review
- Endocytic pathways involved in PLGA nanoparticle uptake by grapevine cells and role of cell wall and membrane in size selection.Plant cell reports · 2017Article
- Article
- Stomatal Closure and Rise in ROS/NO of Arabidopsis Guard Cells by Tobacco Microbial Elicitors: Cryptogein and Harpin.Frontiers in plant science · 2017Article
- Plasma membrane order and fluidity are diversely triggered by elicitors of plant defence.Journal of experimental botany · 2016Article
- SGT1 is required in PcINF1/SRC2-1 induced pepper defense response by interacting with SRC2-1.Scientific reports · 2016Article
- Elicitin-Induced Distal Systemic Resistance in Plants is Mediated Through the Protein-Protein Interactions Influenced by Selected Lysine Residues.Frontiers in plant science · 2016Article
- Loss of Arabidopsis thaliana Dynamin-Related Protein 2B reveals separation of innate immune signaling pathways.PLoS pathogens · 2014Article
- Plasma membrane protein trafficking in plant-microbe interactions: a plant cell point of view.Frontiers in plant science · 2014Article
- Modification of plasma membrane organization in tobacco cells elicited by cryptogein.Plant physiology · 2014Article
- Sensitivity to Flg22 is modulated by ligand-induced degradation and de novo synthesis of the endogenous flagellin-receptor FLAGELLIN-SENSING2.Plant physiology · 2014Article
- Characterization of a gene encoding clathrin heavy chain in maize up-regulated by salicylic acid, abscisic acid and high boron supply.International journal of molecular sciences · 2013Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The plant defense elicitor cryptogein triggers well-known biochemical events of early signal transduction at the plasma membrane of tobacco (Nicotiana tabacum) cells, but microscopic observations of cell responses related to these early events were lacking. We determined that internalization of the lipophilic dye FM4-64, which is a marker of endocytosis, is stimulated a few minutes after addition of cryptogein to tobacco Bright Yellow-2 (BY-2) cells. This stimulation is specific to the signal transduction pathway elicited by cryptogein because a lipid transfer protein, which binds to the same receptor as cryptogein but without triggering signaling, does not increase endocytosis. To define the nature of the stimulated endocytosis, we quantified clathrin-coated pits (CCPs) forming on the plasma membrane of BY-2 cells. A transitory stimulation of this morphological event by cryptogein occurs within the first 15 min. In the presence of cryptogein, increases in both FM4-64 internalization and clathrin-mediated endocytosis are specifically blocked upon treatment with 5 microm tyrphostin A23, a receptor-mediated endocytosis inhibitor. The kinetics of the transient increase in CCPs at the plasma membrane coincides with that of transitory reactive oxygen species (ROS) production occurring within the first 15 min after elicitation. Moreover, in BY-2 cells expressing NtrbohD antisense cDNA, which are unable to produce ROS when treated with cryptogein, the CCP stimulation is inhibited. These results indicate that the very early endocytic process induced by cryptogein in tobacco is due, at least partly, to clathrin-mediated endocytosis and is dependent on ROS production by the NADPH oxidase NtrbohD.
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