ArticleMolecular biotechnology2008
Agrobacterium-mediated transformation and insertional mutagenesis in Colletotrichum acutatum for investigating varied pathogenicity lifestyles.
Article in Molecular biotechnology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed.
- Morphological, Pathological and Genetic Diversity of theJournal of fungi (Basel, Switzerland) · 2022Article
- Inhibition of AoAur1 increases mycelial growth, hyphal fusion and improves physiological adaptation to high-temperature stress in Aspergillus oryzae.Archives of microbiology · 2022Article
- Protoplast-mediated transformation in Sporisorium scitamineum facilitates visualization of in planta developmental stages in sugarcane.Molecular biology reports · 2021Article
- A new fungus-mediated RNAi method established and used to study the fatty acid and retinol binding protein function of the plant-parasitic nematodeRNA biology · 2021Article
- CaNRT2.1 Is Required for Nitrate but Not Nitrite Uptake in Chili Pepper PathogenFrontiers in microbiology · 2020Article
- Generation ofMicroorganisms · 2019Article
- Optimization of Polyethylene Glycol-Mediated Transformation of the Pepper Anthracnose PathogenThe plant pathology journal · 2019Article
- A new and efficient approach for construction of uridine/uracil auxotrophic mutants in the filamentous fungus Aspergillus oryzae using Agrobacterium tumefaciens-mediated transformation.World journal of microbiology & biotechnology · 2017Article
- Article
- The construction and use of versatile binary vectors carrying pyrG auxotrophic marker and fluorescent reporter genes for Agrobacterium-mediated transformation of Aspergillus oryzae.World journal of microbiology & biotechnology · 2016Article
- UvHOG1 is important for hyphal growth and stress responses in the rice false smut fungus Ustilaginoidea virens.Scientific reports · 2016Article
- Article
- Agrobacterium tumefaciens-mediated transformation of Valsa mali: an efficient tool for random insertion mutagenesis.TheScientificWorldJournal · 2013Article
- Functional genomics tools to decipher the pathogenicity mechanisms of the necrotrophic fungus Plectosphaerella cucumerina in Arabidopsis thaliana.Molecular plant pathology · 2013Article
- Identification of pathogenicity-related genes in the vascular wilt fungus Verticillium dahliae by Agrobacterium tumefaciens-mediated T-DNA insertional mutagenesis.Molecular biotechnology · 2011Article
- Identification of virulence genes in the corn pathogen Colletotrichum graminicola by Agrobacterium tumefaciens-mediated transformation.Molecular plant pathology · 2011Article
- Article
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5 authors.
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Abstract
Colletotrichum acutatum is a cosmopolitan pathogen causing economically important diseases known as anthracnose on a wide range of hosts. This fungus exhibits varied pathogenicity lifestyles and the tools essential to understand the molecular mechanisms are still being developed. The transformation methods currently available for this species for gene discovery and functional analysis involve protoplast transformation and are laborious and inefficient. We have developed a protocol for efficient Agrobacterium tumefaciens-mediated transformation (ATMT) of C. acutatum. Using this protocol we were able to transform C. acutatum isolates belonging to different genetic groups and originating from different hosts. The transformation efficiency was up to 156 transformants per 10(4) conidia, with >70% transformants showing single location/single copy integration of T-DNA. Binary vector pBHt2-GFP was constructed, enabling green fluorescence protein tagging of C. acutatum strains, which will be a useful tool for epidemiology and histopathology studies. The ATMT protocol developed was used to identify putative pathogenicity mutants, suggesting the applicability of this technique for rapid generation of a large panel of insertional mutants of C. acutatum leading to the identification of the genes associated with the varied lifestyles.
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