ArticlePloS one2008
The Drosophila cytosine-5 methyltransferase Dnmt2 is associated with the nuclear matrix and can access DNA during mitosis.
Article in PloS one, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 49 citations in OpenAlex.
- Review
- Characterization of the dual role of Plasmodium falciparum DNA methyltransferase in regulating transcription and translation.Nucleic acids research · 2023Article
- RNA 5-methylcytosine status is associated with DNMT2/TRDMT1 nuclear localization in osteosarcoma cell lines.Journal of bone oncology · 2022Article
- Malaria Parasite Stress Tolerance Is Regulated by DNMT2-Mediated tRNA Cytosine Methylation.mBio · 2021Article
- Ubiquitination-mediated degradation of TRDMT1 regulates homologous recombination and therapeutic response.NAR cancer · 2021Article
- Review
- Article
- Mechanism and biological role of Dnmt2 in Nucleic Acid Methylation.RNA biology · 2017Review
- Wolbachia elevates host methyltransferase expression to block an RNA virus early during infection.PLoS pathogens · 2017Article
- Genome-Wide Analysis of Differentially Expressed microRNA in Bombyx mori Infected with Nucleopolyhedrosis Virus.PloS one · 2016Article
- NSUN6 is a human RNA methyltransferase that catalyzes formation of m5C72 in specific tRNAs.RNA (New York, N.Y.) · 2015Article
- DNA methyltransferase 1 mutations and mitochondrial pathology: is mtDNA methylated?Frontiers in genetics · 2015Article
- Article
- The mysterious presence of a 5-methylcytosine oxidase in the Drosophila genome: possible explanations.Cell cycle (Georgetown, Tex.) · 2013Article
- Wolbachia uses a host microRNA to regulate transcripts of a methyltransferase, contributing to dengue virus inhibition in Aedes aegypti.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Efficient RNA virus control in Drosophila requires the RNA methyltransferase Dnmt2.EMBO reports · 2013Article
- Unexplored potentials of epigenetic mechanisms of plants and animals-theoretical considerations.Genetics & epigenetics · 2013Review
- Epigenetic mechanisms of genomic imprinting: common themes in the regulation of imprinted regions in mammals, plants, and insects.Genetics research international · 2012Article
- 5-methylcytosine in RNA: detection, enzymatic formation and biological functions.Nucleic acids research · 2010Review
- Solving the Dnmt2 enigma.Chromosoma · 2010Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytosine-5 methyltransferases of the Dnmt2 family are highly conserved in evolution and their biological function is being studied in several organisms. Although all structural DNA methyltransferase motifs are present in Dnmt2, these enzymes show a strong tRNA methyltransferase activity. In line with an enzymatic activity towards substrates other than DNA, Dnmt2 has been described to localize to the cytoplasm. Using molecular and biochemical approaches we show here that Dnmt2 is both a cytoplasmic and a nuclear protein. Sub-cellular fractionation shows that a significant amount of Dnmt2 is bound to the nuclear matrix. Sub-cellular localization analysis reveals that Dnmt2 proteins are enriched in actively dividing cells. Dnmt2 localization is highly dynamic during the cell cycle. Using live imaging we observed that Dnmt2-EGFP enters prophase nuclei and shows a spindle-like localization pattern during mitotic divisions. Additional experiments suggest that this localization is microtubule dependent and that Dnmt2 can access DNA during mitotic cell divisions. Our results represent the first comprehensive characterization of Dnmt2 proteins on the cellular level and have important implications for our understanding of the molecular activities of Dnmt2.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.