ArticleMolecular microbiology2008
A polymerase III-like reinitiation mechanism is operating in regulation of histone expression in archaea.
Article in Molecular microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 46 citations in OpenAlex.
- Transcription termination and readthrough in African swine fever virus.Frontiers in immunology · 2024Article
- aCPSF1 cooperates with terminator U-tract to dictate archaeal transcription termination efficacy.eLife · 2021Article
- Gene Architecture Facilitates Intron-Mediated Enhancement of Transcription.Frontiers in molecular biosciences · 2021Review
- The conserved ribonuclease aCPSF1 triggers genome-wide transcription termination of Archaea via a 3'-end cleavage mode.Nucleic acids research · 2020Article
- Identification of RNA 3´ ends and termination sites inRNA biology · 2020Article
- FttA is a CPSF73 homologue that terminates transcription in Archaea.Nature microbiology · 2020Article
- Archaeal physiology: The secrets of termination.Nature microbiology · 2016Article
- Widespread formation of alternative 3' UTR isoforms via transcription termination in archaea.Nature microbiology · 2016Article
- Transcription Regulation in Archaea.Journal of bacteriology · 2016Review
- Manipulating archaeal systems to permit analyses of transcription elongation-termination decisions in vitro.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Investigating transcription reinitiation through in vitro approaches.Transcription · 2014Review
- Comparative overview of RNA polymerase II and III transcription cycles, with focus on RNA polymerase III termination and reinitiation.Transcription · 2014Review
- The RNA polymerase trigger loop functions in all three phases of the transcription cycle.Nucleic acids research · 2013Article
- Recombinogenic properties of Pyrococcus furiosus strain COM1 enable rapid selection of targeted mutants.Applied and environmental microbiology · 2012Article
- Role for gene looping in intron-mediated enhancement of transcription.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- A nexus for gene expression-molecular mechanisms of Spt5 and NusG in the three domains of life.Journal of molecular biology · 2012Review
- Evolution of two modes of intrinsic RNA polymerase transcript cleavage.The Journal of biological chemistry · 2011Article
- Natural competence in the hyperthermophilic archaeon Pyrococcus furiosus facilitates genetic manipulation: construction of markerless deletions of genes encoding the two cytoplasmic hydrogenases.Applied and environmental microbiology · 2011Article
- Evolution of multisubunit RNA polymerases in the three domains of life.Nature reviews. Microbiology · 2011Review
- Transcription termination in the plasmid/virus hybrid pSSVx from Sulfolobus islandicus.Extremophiles : life under extreme conditions · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An archaeal histone gene from the hyperthermophile Pyrococcus furiosus containing four consecutive putative oligo-dT terminator sequences was used as a model system to investigate termination signals and the mechanism of termination in vitro. The archaeal RNA polymerase terminated with high efficiency at the first terminator at 90 degrees C when it contained five to six T residues, at 80 degrees C readthrough was significantly increased. A putative hairpin structure upstream of the first terminator had no effect on termination efficiency. Template competition experiments starting with RNA polymerase molecules engaged in ternary complexes revealed recycling of RNA polymerase from the terminator to the promoter of the same template. This facilitated reinitiation was dependent upon the presence of a terminator sequence suggesting that pausing at the terminator is required for recycling as in the RNA polymerase III system. Replacement of the sequences immediately downstream of the oligo-dT terminator by an AT-rich segment improved termination efficiency. Both AT-rich and GC-rich downstream sequences seemed to impair the facilitated reinitiation pathway. Our data suggest that recycling is dependent on a subtle interplay of pausing of RNA polymerase at the terminator and RNA polymerase translocation beyond the oligo-dT termination signal that is dramatically affected by downstream sequences.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.