ArticlePflugers Archiv : European journal of physiology2008
A structural motif in the C-terminal tail of slo1 confers carbon monoxide sensitivity to human BK Ca channels.
Article in Pflugers Archiv : European journal of physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 52 citations in OpenAlex.
- Review
- Hypoxic Regulation of the Large-Conductance, Calcium and Voltage-Activated Potassium Channel, BK.Frontiers in physiology · 2021Review
- The Large-Conductance, Calcium-Activated Potassium Channel: A Big Key Regulator of Cell Physiology.Frontiers in physiology · 2021Review
- ET-CORM Mediated Vasorelaxation of Small Mesenteric Arteries: Involvement of Kv7 Potassium Channels.Frontiers in pharmacology · 2021Article
- Gas Signaling Molecules and Mitochondrial Potassium Channels.International journal of molecular sciences · 2018Review
- Acute oxygen sensing by the carotid body: a rattlebag of molecular mechanisms.The Journal of physiology · 2018Review
- Carbon monoxide in lung cell physiology and disease.American journal of physiology. Cell physiology · 2018Review
- Is Carotid Body Physiological OFrontiers in physiology · 2018Review
- CO-independent modification of KEuropean journal of pharmacology · 2017Article
- Mechanistic insight into the heme-independent interplay between iron and carbon monoxide in CFTR and Slo1 BKMetallomics : integrated biometal science · 2017Review
- Functional Interactions between BKOxidative medicine and cellular longevity · 2016Article
- Carbon Monoxide Modulates Connexin Function through a Lipid Peroxidation-Dependent Process: A Hypothesis.Frontiers in physiology · 2016Article
- Diverse mechanisms underlying the regulation of ion channels by carbon monoxide.British journal of pharmacology · 2015Review
- BK channels: multiple sensors, one activation gate.Frontiers in physiology · 2015Review
- Big Potassium (BK) ion channels in biology, disease and possible targets for cancer immunotherapy.International immunopharmacology · 2014Review
- Carbon monoxide--physiology, detection and controlled release.Chemical communications (Cambridge, England) · 2014Review
- Heme sensor proteins.The Journal of biological chemistry · 2013Review
- The haptoglobin-CD163-heme oxygenase-1 pathway for hemoglobin scavenging.Oxidative medicine and cellular longevity · 2013Review
- Therapeutic potential of heme oxygenase-1/carbon monoxide in lung disease.International journal of hypertension · 2012Article
- Carbon monoxide: an emerging regulator of ion channels.The Journal of physiology · 2011Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Carbon monoxide (CO) is a potent activator of large conductance, calcium-dependent potassium (BK Ca) channels of vascular myocytes and carotid body glomus cells or when heterologously expressed. Using the human BK Ca channel alpha1-subunit (hSlo1; KCNMA1) stably and transiently expressed in human embryonic kidney 293 cells, the mechanism and structural basis of channel activation by CO was investigated in inside-out, excised membrane patches. Activation by CO was concentration dependent (EC50 approximately 20 microM), rapid, reversible, and evoked a shift in the V 0.5 of -20 mV. CO evoked no changes in either single channel conductance or in deactivation rate but augmented channel activation rate. Activation was independent of the redox state of the channel, or associated compounds/protein partners, and was partially dependent on [Ca2+]i in the physiological range (100-1,000 nM). Importantly, CO "super-stimulated" BK Ca activity even in saturating [Ca2+]i. Single or double mutation of two histidine residues previously implicated in CO sensing did not suppress CO activation but replacing the S9-S10 module of the C-terminal of Slo1 with that of Slo3 completely prevented the action of CO. These findings show that a motif in the S9-S10 part of the C-terminal is essential for CO activation and suggest that this gas transmitter activates the BK Ca channel by redox-independent changes in gating.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.