ArticleIntensive care medicine2008
Ficoll-separated mononuclear cells from sepsis patients are contaminated with granulocytes.
Article in Intensive care medicine, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Multimodal single-cell protein and RNA profiling unveils dysregulated immature neutrophil dynamics in gestational diabetes mellitus.Communications biology · 2026Article
- The Simple prEservatioN of Single cElls method for cryopreservation enables the generation of single-cell immune profiles from whole blood.Frontiers in immunology · 2023Article
- Immune Subsets From Ficoll Density Gradient Separation in Kidney Transplant Recipients.Transplantation direct · 2022Article
- Low-Density Granulocyte Contamination From Peripheral Blood Mononuclear Cells of Patients With Sepsis and How to Remove It - A Technical Report.Frontiers in immunology · 2021Article
- On the origin of low-density neutrophils.Journal of leukocyte biology · 2020Review
- Fibrocytes are increased in lung and peripheral blood of patients with idiopathic pulmonary fibrosis.Respiratory research · 2018Article
- Neutrophils with myeloid derived suppressor function deplete arginine and constrain T cell function in septic shock patients.Critical care (London, England) · 2014Article
- Endothelium dependent vasomotion and in vitro markers of endothelial repair in patients with severe sepsis: an observational study.PloS one · 2013Observational
- Immunomagnetic bead separation of mononuclear cells from contaminating granulocytes in cryopreserved blood samples.Cryobiology · 2009Article
- Poor functional recovery after transplantation of diabetic bone marrow stem cells in ischemic myocardium.The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation · 2009Article
- Glucocorticoid receptor mRNA levels are selectively decreased in neutrophils of children with sepsis.Intensive care medicine · 2009Article
- Year in review in Intensive Care Medicine, 2008: II. Experimental, acute respiratory failure and ARDS, mechanical ventilation and endotracheal intubation.Intensive care medicine · 2009Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo determine the cell content and purity of Ficoll-separated peripheral blood mononuclear cells and granulocyte isolates in sepsis patients compared to healthy controls. DESIGN AND
settingProspective study in the adult and pediatric intensive care departments of the Erasmus University Medical Center in the Netherlands. PATIENTS: Three sepsis patients (two adults, one child) and four healthy controls. MEASUREMENTS AND
resultsBlood leukocytes were separated by Ficoll into an interface and a bottom fraction. The cell content and purity was analyzed by cytospin and flow-cytometric immunofluorescence. In sepsis patients, the interface consisted of 11-52% mononuclear cells only, due to high contamination with granulocytes (48-89%). This was in contrast to a high proportion of mononuclear cells (88-100%) in healthy controls. The bottom fraction showed a cell purity of >or=92% polymorphonuclear granulocytes in sepsis patients as well as in healthy controls.
conclusionsFicoll-separated leukocytes of sepsis patients are not suitable for studying mononuclear cells but can be used for studying granulocytes with high purity. The mononuclear cell fraction is highly contaminated with granulocytes. Additional separation techniques are necessary to obtain a pure cell fraction.
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