ArticleMolecular therapy : the journal of the American Society of Gene Therapy2008
Quantitative comparison of intracellular unpacking kinetics of polyplexes by a model constructed from quantum dot-FRET.
Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
56 citing papers in PubMed, 151 citations in OpenAlex.
- Stimulus-Responsive Polymeric Carriers for Gene Delivery: Balancing Endosomal Escape with Nucleic Acid Release.Pharmaceutical research · 2026Review
- Achieving Maximum Efficiency of A549 Cell Transfection with a Plasmid Delivered by Polyethylenimine-Based Polyplexes.Doklady. Biochemistry and biophysics · 2026Article
- Closing the Gap between Experiment and Simulation─A Holistic Study on the Complexation of Small Interfering RNAs with Polyethylenimine.Molecular pharmaceutics · 2024Article
- Polymeric-Micelle-Based Delivery Systems for Nucleic Acids.Pharmaceutics · 2023Review
- Engineering poly- and micelleplexes for nucleic acid delivery - A reflection on their endosomal escape.Journal of controlled release : official journal of the Controlled Release Society · 2023Review
- Article
- Quinine copolymer reporters promote efficient intracellular DNA delivery and illuminate a protein-induced unpackaging mechanism.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Photosensitive Poly-l-lysine/Heparin Interpolyelectrolyte Complexes for Delivery of Genetic Drugs.Polymers · 2020Article
- A facile methodology using quantum dot multiplex labels for tracking co-transfection.RSC advances · 2019Article
- Predicting Gene Silencing Through the Spatiotemporal Control of siRNA Release from Photo-responsive Polymeric Nanocarriers.Journal of visualized experiments : JoVE · 2017Article
- Anionic Polymer and Quantum Dot Excipients to Facilitate siRNA Release and Self-Reporting of Disassembly in Stimuli-Responsive Nanocarrier Formulations.Biomacromolecules · 2017Article
- Surface engineering for lymphocyte programming.Advanced drug delivery reviews · 2017Review
- Efficient tuning of siRNA dose response by combining mixed polymer nanocarriers with simple kinetic modeling.Acta biomaterialia · 2017Article
- Effect of lipid headgroup charge and pH on the stability and membrane insertion potential of calcium condensed gene complexes.Langmuir : the ACS journal of surfaces and colloids · 2015Article
- Current advances in polymer-based nanotheranostics for cancer treatment and diagnosis.ACS applied materials & interfaces · 2014Review
- Insight into mechanisms of cellular uptake of lipid nanoparticles and intracellular release of small RNAs.Pharmaceutical research · 2014Article
- Location deterministic biosensing from quantum-dot-nanowire assemblies.Applied physics letters · 2014Article
- Bioreducible polycations in nucleic acid delivery: past, present, and future trends.Macromolecular bioscience · 2014Review
- Quantum dots in diagnostics and detection: principles and paradigms.The Analyst · 2014Review
- Effect of polyplex morphology on cellular uptake, intracellular trafficking, and transgene expression.ACS nano · 2013Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
A major challenge for non-viral gene delivery is gaining a mechanistic understanding of the rate-limiting steps. A critical barrier in polyplex-mediated gene delivery is the timely unpacking of polyplexes within the target cell to liberate DNA for efficient gene transfer. In this study, the component plasmid DNA and polymeric gene carrier were individually labeled with quantum dots (QDs) and Cy5 dyes, respectively, as a donor and acceptor pair for fluorescence resonance energy transfer (FRET). The high signal-to-noise ratio in QD-mediated FRET enabled sensitive detection of discrete changes in polyplex stability. The intracellular uptake and dissociation of polyplexes through QD-FRET was captured over time by confocal microscopy. From quantitative image-based analysis, distributions of released plasmid within the endo/lysosomal, cytosolic, and nuclear compartments formed the basis for constructing a three-compartment first-order kinetics model. Polyplex unpacking kinetics for chitosan, polyethylenimine, and polyphosphoramidate were compared and found to correlate well with transfection efficiencies. Thus, QD-FRET-enabled detection of polyplex stability combined with image-based quantification is a valuable method for studying mechanisms involved in polyplex unpacking and trafficking within live cells. We anticipate that this method will also aid the design of more efficient gene carriers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.