ArticleJournal of the American Chemical Society2008
Using ion channel-forming peptides to quantify protein-ligand interactions.
Article in Journal of the American Chemical Society, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 51 citations in OpenAlex.
- Multi-oligomeric states of alamethicin ion channel: Assemblies and conductance.Biophysical journal · 2023Article
- Assembly of transmembrane pores from mirror-image peptides.Nature communications · 2022Article
- Designed alpha-helical barrels for charge-selective peptide translocation.Chemical science · 2020Article
- Building membrane nanopores.Nature nanotechnology · 2017Review
- Enhanced Temporal Resolution with Ion Channel-Functionalized Sensors Using a Conductance-Based Measurement Protocol.Analytical chemistry · 2017Article
- Monitoring charge flux to quantify unusual ligand-induced ion channel activity for use in biological nanopore-based sensors.Analytical chemistry · 2014Article
- Watching single proteins using engineered nanopores.Protein and peptide letters · 2014Review
- Multivariate analyses of amyloid-beta oligomer populations indicate a connection between pore formation and cytotoxicity.PloS one · 2012Article
- Applications of biological pores in nanomedicine, sensing, and nanoelectronics.Current opinion in biotechnology · 2010Review
- Metal-assisted channel stabilization: disposition of a single histidine on the N-terminus of alamethicin yields channels with extraordinarily long lifetimes.Biophysical journal · 2010Article
- A semi-synthetic ion channel platform for detection of phosphatase and protease activity.ACS nano · 2009Article
- Gramicidin pores report the activity of membrane-active enzymes.Journal of the American Chemical Society · 2009Article
- Amyloid-beta-induced ion flux in artificial lipid bilayers and neuronal cells: resolving a controversy.Neurotoxicity research · 2009Article
- Carbonic anhydrase as a model for biophysical and physical-organic studies of proteins and protein-ligand binding.Chemical reviews · 2008Review
- Methacrylate Polymer Scaffolding Enhances the Stability of Suspended Lipid Bilayers for Ion Channel Recordings and Biosensor Development.ACS biomaterials science & engineeringArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
This paper proposes a method for sensing affinity interactions by triggering disruption of self-assembly of ion channel-forming peptides in planar lipid bilayers. It shows that the binding of a derivative of alamethicin carrying a covalently attached sulfonamide ligand to carbonic anhydrase II (CA II) resulted in the inhibition of ion channel conductance through the bilayer. We propose that the binding of the bulky CA II protein (MW approximately 30 kD) to the ion channel-forming peptides (MW approximately 2.5 kD) either reduced the tendency of these peptides to self-assemble into a pore or extracted them from the bilayer altogether. In both outcomes, the interactions between the protein and the ligand lead to a disruption of self-assembled pores. Addition of a competitive inhibitor, 4-carboxybenzenesulfonamide, to the solution released CA II from the alamethicin-sulfonamide conjugate and restored the current flow across the bilayer by allowing reassembly of the ion channels in the bilayer. Time-averaged recordings of the current over discrete time intervals made it possible to quantify this monovalent ligand binding interaction. This method gave a dissociation constant of approximately 2 microM for the binding of CA II to alamethicin-sulfonamide in the bilayer recording chamber: this value is consistent with a value obtained independently with CA II and a related sulfonamide derivative by isothermal titration calorimetry.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.