ArticleBiophysical journal2008
A 20-nm step toward the cell membrane preceding exocytosis may correspond to docking of tethered granules.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Computational design of bifaceted protein nanomaterials.Nature materials · 2025Article
- Vesicle docking and fusion pore modulation by the neuronal calcium sensor Synaptotagmin-1.Biophysical journal · 2025Article
- Vesicle docking and fusion pore modulation by the neuronal calcium sensor Synaptotagmin-1.bioRxiv : the preprint server for biology · 2024Article
- VAMP2 and synaptotagmin mobility in chromaffin granule membranes: implications for regulated exocytosis.Molecular biology of the cell · 2022Article
- Toward a unified picture of the exocytotic fusion pore.FEBS letters · 2018Review
- High Throughput NPY-Venus and Serotonin Secretion Assays for Regulated Exocytosis in Neuroendocrine Cells.Bio-protocol · 2018Article
- BAIAP3, a C2 domain-containing Munc13 protein, controls the fate of dense-core vesicles in neuroendocrine cells.The Journal of cell biology · 2017Article
- Reconstitution of calcium-mediated exocytosis of dense-core vesicles.Science advances · 2017Article
- A Programmable DNA Origami Platform to Organize SNAREs for Membrane Fusion.Journal of the American Chemical Society · 2016Article
- Resident CAPS on dense-core vesicles docks and primes vesicles for fusion.Molecular biology of the cell · 2016Article
- Article
- Cdc42 controls the dilation of the exocytotic fusion pore by regulating membrane tension.Molecular biology of the cell · 2014Article
- Properties of ribbon and non-ribbon release from rod photoreceptors revealed by visualizing individual synaptic vesicles.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Myrip couples the capture of secretory granules by the actin-rich cell cortex and their attachment to the plasma membrane.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Article
- Interactive, computer-assisted tracking of speckle trajectories in fluorescence microscopy: application to actin polymerization and membrane fusion.Biophysical journal · 2011Article
- Mapping dynamic protein interactions to insulin secretory granule behavior with TIRF-FRET.Biophysical journal · 2010Article
- Quantitative analysis of the native presynaptic cytomatrix by cryoelectron tomography.The Journal of cell biology · 2010Article
- Differential Regulation of Synaptic Vesicle Tethering and Docking by UNC-18 and TOM-1.Frontiers in synaptic neuroscience · 2010Article
- Model of SNARE-mediated membrane adhesion kinetics.PloS one · 2009Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In endocrine cells, plasma membrane (PM)-bound secretory granules must undergo a number of maturation stages (i.e., priming) to become fusion-competent. Despite identification of several molecules involved in binding granules to the PM and priming them, the exact nature of events occurring at the PM still largely remains a mystery. In stimulated BON cells, we used evanescent wave microscopy to study trajectories of granules shortly before their exocytoses, which provided a physical description of vesicle-PM interactions at an unprecedented level of detail, and directly lead to an original mechanistic model. In these cells, tethered (T), nonfusogenic, vesicles are prevented from converting to fusogenic, docked (D) ones in resting conditions. Upon elevation of calcium, T-vesicles perform a 21-nm step toward the PM to become D, and fuse approximately 3 s thereafter. Our ability to directly visualize different modes of PM-attachment paves the way for clarifying the exact role of various molecules implicated in attachment and priming of granules in future studies.
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