ArticlePhotosynthesis research2008
Purification of His6-tagged Photosystem I from Chlamydomonas reinhardtii.
Article in Photosynthesis research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 33 citations in OpenAlex.
- Development of a Novel Nanoarchitecture of the Robust Photosystem I from a Volcanic MicroalgaInternational journal of molecular sciences · 2021Article
- Expression and purification of affinity-tagged variants of the photochemical reaction center from Heliobacterium modesticaldum.Photosynthesis research · 2019Article
- Four distinct trimeric forms of light-harvesting complex II isolated from the green alga Chlamydomonas reinhardtii.Photosynthesis research · 2019Article
- Structure of a PSI-LHCI-cyt bProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Uphill energy transfer in photosystem I from Chlamydomonas reinhardtii. Time-resolved fluorescence measurements at 77 K.Photosynthesis research · 2018Article
- STATE TRANSITION7-Dependent Phosphorylation Is Modulated by Changing Environmental Conditions, and Its Absence Triggers Remodeling of Photosynthetic Protein Complexes.Plant physiology · 2015Article
- Spontaneous activation of [FeFe]-hydrogenases by an inorganic [2Fe] active site mimic.Nature chemical biology · 2013Article
- Energy-dissipative supercomplex of photosystem II associated with LHCSR3 in Chlamydomonas reinhardtii.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- A dual strategy to cope with high light in Chlamydomonas reinhardtii.The Plant cell · 2013Article
- Photosystem I of Chlamydomonas reinhardtii contains nine light-harvesting complexes (Lhca) located on one side of the core.The Journal of biological chemistry · 2011Article
- Efficient light harvesting in a dark, hot, acidic environment: the structure and function of PSI-LHCI from Galdieria sulphuraria.Biophysical journal · 2011Article
- Isolation of the elusive supercomplex that drives cyclic electron flow in photosynthesis.Nature · 2010Article
- Requirements for construction of a functional hybrid complex of photosystem I and [NiFe]-hydrogenase.Applied and environmental microbiology · 2010Article
- PSI-LHCI of Chlamydomonas reinhardtii: Increasing the absorption cross section without losing efficiency.Biochimica et biophysica actaArticle
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a rapid method for isolation of the Photosystem I (PS1) complex from Chlamydomonas reinhardtii using epitope tagging. Six histidine residues were genetically added to the N-terminus of the PsaA core subunit of PS1. The His(6)-tagged PS1 could be purified with a yield of 80-90% from detergent-solubilized thylakoid membranes within 3 h in a single step using a Ni-nitrilotriacetic acid (Ni-NTA) column. Immunoblots and low-temperature fluorescence analysis indicated that the His(6)-tagged PS1 preparation was highly pure and extremely low in uncoupled pigments. Moreover, the introduced tag appeared to have no adverse effect upon PS1 structure/function, as judged by photochemical assays and EPR spectroscopy of isolated particles, as well as photosynthetic growth tests of the tagged strain.
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