ArticleProceedings of the National Academy of Sciences of the United States of America2008
X-ray absorption spectroscopic characterization of a cytochrome P450 compound II derivative.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Correlating Structure with Spectroscopy in Ascorbate Peroxidase Compound II.Journal of the American Chemical Society · 2024Article
- Heme-copper and Heme OJournal of inorganic biochemistry · 2023Review
- An S=1 Iron(IV) Intermediate Revealed in a Non-Heme Iron Enzyme-Catalyzed Oxidative C-S Bond Formation.Angewandte Chemie (International ed. in English) · 2023Article
- Article
- Aspartate or arginine? Validated redox state X-ray structures elucidate mechanistic subtleties of FeJournal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2021Review
- Heme-FeJournal of the American Chemical Society · 2020Article
- X-ray snapshots reveal conformational influence on active site ligation during metalloprotein folding.Chemical science · 2019Article
- Reaction Intermediates and Molecular Mechanism of Peroxynitrite Activation by NO Synthases.Biophysical journal · 2016Article
- Mechanistic insight from thermal activation parameters for oxygenation reactions of different substrates with biomimetic iron porphyrin models for compounds I and II.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2012Article
- Prediction of high-valent iron K-edge absorption spectra by time-dependent density functional theory.Dalton transactions (Cambridge, England : 2003) · 2011Article
- Molecular probes of the mechanism of cytochrome P450. Oxygen traps a substrate radical intermediate.Archives of biochemistry and biophysics · 2011Article
- Nature of the ferryl heme in compounds I and II.The Journal of biological chemistry · 2011Article
- Low temperature photo-oxidation of chloroperoxidase Compound II.Journal of inorganic biochemistry · 2010Article
- Structure of cytochrome P450 PimD suggests epoxidation of the polyene macrolide pimaricin occurs via a hydroperoxoferric intermediate.Chemistry & biology · 2010Article
- Reaction of N-hydroxyguanidine with the ferrous-oxy state of a heme peroxidase cavity mutant: a model for the reactions of nitric oxide synthase.Archives of biochemistry and biophysics · 2010Article
- Mechanisms of peroxynitrite interactions with heme proteins.Inorganic chemistry · 2010Article
- Bioinspired heme, heme/nonheme diiron, heme/copper, and inorganic NOx chemistry: *NO((g)) oxidation, peroxynitrite-metal chemistry, and *NO((g)) reductive coupling.Inorganic chemistry · 2010Article
- Hydrocarbon hydroxylation by cytochrome P450 enzymes.Chemical reviews · 2010Review
- Kinetics and activation parameters for oxidations of styrene by Compounds I from the cytochrome P450(BM-3) (CYP102A1) heme domain and from CYP119.Biochemistry · 2009Article
- Mechanistic aspects of CYP74 allene oxide synthases and related cytochrome P450 enzymes.Phytochemistry · 2009Review
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6 authors.
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Abstract
The cytochrome P450 enzyme CYP119, its compound II derivative, and its nitrosyl complex were studied by iron K-edge x-ray absorption spectroscopy. The compound II derivative was prepared by reaction of the resting enzyme with peroxynitrite and had a lifetime of approximately 10 s at 23 degrees C. The CYP119 nitrosyl complex was prepared by reaction of the enzyme with nitrogen monoxide gas or with a nitrosyl donor and was stable at 23 degrees C for hours. Samples of CYP119 and its derivatives were studied by x-ray absorption spectroscopy at temperatures below 140 (K) at the Advanced Photon Source of Argonne National Laboratory. The x-ray absorption near-edge structure spectra displayed shifts in edge and pre-edge energies consistent with increasing effective positive charge on iron in the series native CYP119 < CYP119 nitrosyl complex < CYP119 compound II derivative. Extended x-ray absorption fine structure spectra were simulated with good fits for k = 12 A(-1) for native CYP119 and k = 13 A(-1) for both the nitrosyl complex and the compound II derivative. The important structural features for the compound II derivative were an iron-oxygen bond length of 1.82 A and an iron-sulfur bond length of 2.24 A, both of which indicate an iron-oxygen single bond in a ferryl-hydroxide, Fe(IV)OH, moiety.
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