ArticleJournal of clinical microbiology2008
Rapid identification of mycobacteria by Raman spectroscopy.
Article in Journal of clinical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Species-level identification of Nocardia spp. from clinical samples via intelligent analysis of Raman spectroscopic fingerprints.World journal of microbiology & biotechnology · 2026Article
- Future Research Directions on Mycobacterium tuberculosis Proteins.Advances in experimental medicine and biology · 2026Review
- Illuminating the Tiny World: A Navigation Guide for Proper Raman Studies on Microorganisms.Molecules (Basel, Switzerland) · 2024Review
- Machine learning analysis of SERS fingerprinting for the rapid determination ofComputational and structural biotechnology journal · 2022Article
- Biofilms of the non-tuberculousCell surface (Amsterdam, Netherlands) · 2020Article
- Identification of mycolic acid forms using surface-enhanced Raman scattering as a fast detection method for tuberculosis.International journal of nanomedicine · 2018Article
- Label-free optical vibrational spectroscopy to detect the metabolic state of M. tuberculosis cells at the site of disease.Scientific reports · 2017Article
- Accurate and Rapid Differentiation of Acinetobacter baumannii Strains by Raman Spectroscopy: a Comparative Study.Journal of clinical microbiology · 2017Article
- Evaluation of antibiotic effects on Pseudomonas aeruginosa biofilm using Raman spectroscopy and multivariate analysis.Biomedical optics express · 2014Article
- Discrimination of Aspergillus lentulus from Aspergillus fumigatus by Raman spectroscopy and MALDI-TOF MS.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2014Article
- Raman spectroscopy provides a powerful, rapid diagnostic tool for the detection of tuberculous meningitis in ex vivo cerebrospinal fluid samples.Journal of biophotonics · 2013Article
- Detecting and tracking nosocomial methicillin-resistant Staphylococcus aureus using a microfluidic SERS biosensor.Analytical chemistry · 2013Article
- Evidence for phenotypic plasticity among multihost Campylobacter jejuni and C. coli lineages, obtained using ribosomal multilocus sequence typing and Raman spectroscopy.Applied and environmental microbiology · 2013Article
- Use of Raman spectroscopy for identification of compatible solutes in halophilic bacteria.Extremophiles : life under extreme conditions · 2012Article
- Clinical manifestations, diagnosis, and treatment of Mycobacterium haemophilum infections.Clinical microbiology reviews · 2011Review
- Towards a point-of-care test for active tuberculosis: obstacles and opportunities.Nature reviews. Microbiology · 2011Review
- Evaluation of Escherichia coli cell response to antibiotic treatment by use of Raman spectroscopy with laser tweezers.Journal of clinical microbiology · 2010Article
- Proof of principle for successful characterization of methicillin-resistant coagulase-negative staphylococci isolated from skin by use of Raman spectroscopy and pulsed-field gel electrophoresis.Journal of clinical microbiology · 2010Article
- Advances in microfluidics in combating infectious diseases.Biotechnology advancesReview
- Point-of-care assays for tuberculosis: role of nanotechnology/microfluidics.Biotechnology advancesReview
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A number of rapid identification methods have been developed to improve the accuracy for diagnosis of tuberculosis and to speed up the presumptive identification of Mycobacterium species. Most of these methods have been validated for a limited group of microorganisms only. Here, Raman spectroscopy was compared to 16S rRNA sequencing for the identification of Mycobacterium tuberculosis complex strains and the most frequently found strains of nontuberculous mycobacteria (NTM). A total of 63 strains, belonging to eight distinct species, were analyzed. The sensitivity of Raman spectroscopy for the identification of Mycobacterium species was 95.2%. All M. tuberculosis strains were correctly identified (7 of 7; 100%), as were 54 of 57 NTM strains (94%). The differentiation between M. tuberculosis and NTM was invariably correct for all strains. Moreover, the reproducibility of Raman spectroscopy was evaluated for killed mycobacteria (by heat and formalin) versus viable mycobacteria. The spectra of the heat-inactivated bacteria showed minimal differences compared to the spectra of viable mycobacteria. Therefore, the identification of mycobacteria appears possible without biosafety level 3 precautions. Raman spectroscopy provides a novel answer to the need for rapid species identification of cultured mycobacteria in a clinical diagnostic setting.
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